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Tigit bv421

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TIGIT-BV421 is a fluorochrome-conjugated antibody that binds to the TIGIT (T-cell immunoreceptor with Ig and ITIM domains) protein. TIGIT-BV421 can be used for the detection and analysis of TIGIT-expressing cells in flow cytometry applications.

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Lab products found in correlation

Cd4 fitc, by BioLegend (1 mentions) Pd 1 apc cy7, by BioLegend (1 mentions) Cd4 percp cy5, by BD (1 mentions) Ctla 4 pe cf594, by BD (1 mentions) Cd3 pe cf594, by BD (1 mentions) Ebioscience foxp3 transcription factor staining buffer set, by Thermo Fisher Scientific (1 mentions) Granzyme b apc fire750, by BioLegend (1 mentions) Il 10 bv421, by BioLegend (1 mentions) Cd3 ax700, by BD (1 mentions) Foxp3 ax647, by BD (1 mentions) Cd25 fitc, by BioLegend (1 mentions) Pe isotype control, by BioLegend (1 mentions) Cd3 apc cy7, by BioLegend (1 mentions) Dnam 1 pe, by BioLegend (1 mentions) Facs lsrii flow cytometer, by BD (1 mentions) Cd152 apc, by BioLegend (1 mentions) Nrp1 bv421, by BioLegend (1 mentions) Helios percp cy5, by BioLegend (1 mentions) Gitr pecy7, by BioLegend (1 mentions) Foxp3 pe, by Thermo Fisher Scientific (1 mentions)

3 protocols using tigit bv421

1

Multiparametric Characterization of Immune Cells from Cryopreserved Samples

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Cryopreserved PBMC/cord BMC (CBMC) were thawed, counted, and processed immediately for phenotypic assessment using two staining panels. Panel A consisted of surface staining with Zombie yellow (viability), CD25 FITC (BioLegend), Lag3 PE (Invitrogen), CTLA4 PE-CF594 (BD Biosciences), CD4 PerCP-Cy5.5 (BD Biosciences), CD3 Ax700 (BD Biosciences) followed by fixation and permeabilization using the eBioscience Foxp3 / Transcription Factor Staining Buffer Set (eBioscience). Intracellular staining was then performed with FoxP3 Ax647 (BD Biosciences), Granzyme B APC-fire750 (BioLegend), IL-10 BV421 (BioLegend) and TGFβ PE-Cy7 (BioLegend). Panel B consisted of surface staining with Zombie yellow (viability), CD4 FITC (BioLegend), CD3 PE-CF594 (BD Biosciences), GITR PerCP-Cy5.5 (BioLegend), TNFR2 PE-Cy7 (BioLegend), CD39 Ax700 (R&D Systems), PD1 APC-Cy7 (BioLegend) and TIGIT BV421 (BioLegend). Intracellular staining consisted of FoxP3 Ax647 (BD Biosciences) and IL-35 PE (BioLegend). Analysis was performed using the Galios instrument (Beckman Coulter).
Johnson M., Lazarus S.K., Bennett A.E., Tovar-Salazar A., Robertson C.E., Kofonow J.M., Li S., McCollister B., Nunes M.C., Madhi S.A., Frank D.N, & Weinberg A. (2024). Gut Microbiota and Other Factors Associated With Increased Regulatory T Cells in Hiv-exposed Uninfected Infants. Research Square.
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2

Flow Cytometric Analysis of T-Cell Markers

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Cell surface staining for flow cytometry was performed using the antibodies CD3-APC-Cy7 (Clone: SK7), γδ-PE-Cy7 (Clone: B1), TIGIT-BV421 (Clone: A15153G), and DNAM-1-PE (Clone: 11Aδ) together with a BV421 isotype Control (Clone: G155-178) and a PE isotype Control (Biolegend, San Diego, USA). The Foxp3-Alexaflour 647 (Clone: 236A/E7) fluorescent antibody was stained independently.
Jin Z., Ye W., Lan T., Zhao Y., Liu X., Chen J., Lai J., Chen S., Zhong X, & Wu X. (2020). Characteristic of TIGIT and DNAM-1 Expression on Foxp3+ γδ T Cells in AML Patients. BioMed Research International, 2020, 4612952.
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3

Characterization of Lymph Node Immune Cells

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Ipsilateral draining submandibular and cervical lymph nodes were extracted from recipient mice (n = 6) of each group at the time of euthanization. The single-cell suspension from the lymph nodes was centrifuged and the final cell concentration was adjusted to 1 × 10 7 /mL. The 100-µl cell suspension was taken to the bottom of the flow tube. Surface staining was performed before intracellular staining. CD4-APC/C7 (Biolegend, clone # GK1.5), CD44-BV510 (Biolegend, clone # IM7), CD62-FITC (Biolegend, clone #MEL-14), CD152-APC (Biolegend, clone #UC10-4B9), GITR-PE/Cy7 (Biolegend, clone #DTA-1), Nrp-1-BV421 (Biolegend, clone #3E12), CD25-APC (Biolegend, clone #PC61), CD62L-FITC (Biolegend, clone #MEL-14), TIGIT-BV421 (Biolegend, clone #1G9), CD226-PE/Cy7 (Biolegend, clone #10E5), Helios-PerCP/Cy5.5 (Biolegend, clone #22F6), and Foxp3-PE (Thermo, clone# FJK-16s) were used in this study. Fluorescence-activated cell sorting (FACS) was performed using BD FACS LSRII Flow Cytometer. Fifty thousand lymphocytes for each sample were collected by FACS Diva 8.0 software and finally analyzed by Flowjo software (BD Bioscience, USA).
Li S., Zhang P., Li A., Bao J., Pan Z, & Jie Y. (2023). TIGIT-Fc Prolongs Corneal Allograft Survival in Mice by Upregulating TIGIT/CD226 Expression and the Proportion of Helios + Foxp3 + Treg Cells. Transplantation, 107(2).
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