Model 7025

Manufactured by Ugo Basile

Sourced in Italy

The Model 7025 is a laboratory instrument designed for the determination of the pain threshold and analgesia in small laboratory animals. It features a digital display and allows for the precise measurement of the force applied to the animal's tail or paw.

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Lab products found in correlation

Stereotaxic frame, by Kopf Instruments (1 mentions) Dtx plus dt xx, by BD (1 mentions) Homeothermic blanket system, by Harvard Apparatus (1 mentions) Combowire, by Philips (1 mentions) Combomap, by Philips (1 mentions)

4 protocols using model 7025

Laser-Doppler Flowmetry for Cerebral Blood Flow

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Regional CBF was assessed by means of laser-Doppler flowmetry (ALF21D, Advance Co. Ltd, Tokyo, Japan) before and immediately after MCAO. The laser-Doppler probe was positioned at 2 mm posterior and 4 mm lateral to the bregma above the intact dura as previously described [11] (link). Laser-Doppler flowmetry is a method to estimate the blood perfusion in the microcirculation. Therefore, special attention was paid to avoid confounding effects of superfluous light, heterogeneous distribution of superficial blood vessels, and movement artifacts on laser-Doppler flowmetry [13] . For mechanical ventilation, we used a respirator with little vibration (Rodent ventilator, Model 7025, Ugo Basile, Comerio VA, Italy), which does not affect the laser-Doppler flowmetry by movement artifacts even placed on the experimental table.
In a subgroup of rats, regional CBF was assessed by laser-Doppler flowmetry at points 2 mm posterior and 2.0 to 3.0 mm lateral to the bregma by scanning the laser-Doppler probe at 0.1 mm intervals with a stereotaxic device (Type SR-5N and SM-11, Narishige Scientific Instrument Laboratory, Tokyo, Japan) before and 30 min after distal MCAO (Figure 1A).

Yao H, & Nabika T. (2014). Excess Salt Increases Infarct Size Produced by Photothrombotic Distal Middle Cerebral Artery Occlusion in Spontaneously Hypertensive Rats. PLoS ONE, 9(5), e97109.

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Comprehensive Physiological Monitoring in Rats

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The left femoral artery was cannulated for continuous monitoring of arterial blood pressure using a transducer (DTX Plus DT-XX; Becton Dickinson, Sandy, UT, USA) connected to an amplifier (PM-1000; CWE, Ardmore, PA, USA). After a tracheostomy, the trachea was cannulated and the rats were ventilated (model 7025; Ugo Basile, Comerio, VA, Italy) with oxygen-enriched air (40% v:v, 1.0 L/min, 2.5–3.5 mL per stroke, 80–100 strokes per minute), and end-tidal CO2 was monitored (CapStar-100; CWE) and maintained between 3.5% and 4.5%. The rats were placed on a heating blanket with a DC-power supply designed to emit only extremely low levels of electromagnetic-radiofrequency (EMF-RF) noise interference and thermostatically regulated by continuous feedback from a thermistor circuit (Homeothermic blanket system; Harvard Apparatus, Holliston, MA, USA) to maintain core body temperature at 37 ± 0.5 °C. The rat’s heads were fixed in a stereotaxic frame (Kopf Instruments, Tujunga, CA, USA). Data regarding the variables described above, blood pressure, tidal CO2, and core temperature, was fed to a data acquisition system (Power 1401, CED, Cambridge, UK) and laptop computer where they were continuously displayed and monitored to assist maintenance between physiological limits, and stored on hard disk.

Hoffmann J., Storer R.J., Park J.W, & Goadsby P.J. (2019). N-Methyl-D-aspartate receptor open-channel blockers memantine and magnesium modulate nociceptive trigeminovascular neurotransmission in rats. The European journal of neuroscience, 50(5), 2847-2859.

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Echocardiography and Right Heart Catheterization in Rats

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At the end of the 21-day period, transthoracic echocardiography (TTE; Vevo2100, MS250 linear array probe, Visual Sonics, Toronto, ON, Canada) was performed on a spontaneously breathing rat anesthetized with sevoflurane (induction: 7% and maintenance: 4%) in oxygen. B-mode and pulsed wave Doppler images of the PT were acquired (1 (link)).
Subsequently, anesthetized animals (sevoflurane in a 3:1 mixture of oxygen and nitrous oxide) were ventilated at 75 strokes/min with a tidal volume of 10 ml/kg (model 7025, Ugo Basile, Varese, Italy). Right heart catheterization (RHC) was performed through a left lateral thoracotomy. A 0.014-in. combined dual-tipped pressure and Doppler flow sensor wire (Combowire, Philips Volcano) was inserted through the RV outflow tract into the PT to acquire pressure and velocity data at a sampling rate of 200 Hz (Combomap, Philips Volcano). At the end of the procedure, animals were euthanized by decapitation.

Su J., Logan C.C., Hughes A.D., Parker K.H., Dhutia N.M., Danielsen C.C, & Simonsen U. (2018). Impact of chronic hypoxia on proximal pulmonary artery wave propagation and mechanical properties in rats. American Journal of Physiology - Heart and Circulatory Physiology, 314(6), H1264-H1278.

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Myocardial Infarction Induction in Rodents

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The animals were intubated and mechanically ventilated using a rodent-apparatus (model 7025, Ugo Basile, Comerio, VA, Italy); anesthesia was maintained with a mixture of oxygen and 2.5% sevoflurane. Through a left thoracotomy, the left coronary artery was encircled and ligated 4 mm from its origin with a 6-0 suture, extending from the pulmonary cone to the left atrial appendage, as previously (Kolettis et al., 2007) ; following these anatomical landmarks ensures comparable infarct size (Baltogiannis et al., 2005) . The generation MI was confirmed by the inspection of a pale, akinetic area and by ST elevation in a six-lead ECG (QRS-Card PC-ECG, Pulse Biomedical Inc., PBI, Norristown, PA), after amplification (Cardiology Suite version 4.05, PBI). In sham-operated animals, the left coronary artery was encircled, but not ligated.

Daskalopoulos E.P., Vilaeti A.D., Barka E., Mantzouratou P., Kouroupis D., Kontonika M., Tourmousoglou C., Papalois A., Pantos C., Blankesteijn W.M., Agathopoulos S, & Kolettis T.M. (2015). Attenuation of post-infarction remodeling in rats by sustained myocardial growth hormone administration. Growth factors (Chur, Switzerland), 33(4).

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