HeLa, SiHa and THP-1 cell lines used in this study were obtained from American Type Culture Collection (ATCC, Manassas, VA, USA). THP-1 was grown in PRMI Medium 1640 (Gibco). HeLa and SiHa were cultured in Dulbecco's modified Eagle's medium (Gibco). All medium was supplemented with 10% fetal bovine serum (Gibco) and antibiotics (penicillin and streptomycin) (Gibco). Erastin, RSL3, sorafenib, AS1517499 (STAT6 inhibitor) and STAT3-IN-1 (STAT3 inhibitor) were purchased from MedChemExpress.
Stat3 in 1
Manufactured by MedChemExpress
STAT3-IN-1 is a small molecule inhibitor that targets the STAT3 (Signal Transducer and Activator of Transcription 3) signaling pathway. STAT3 is a transcription factor that plays a crucial role in cellular processes such as cell growth, survival, and differentiation. STAT3-IN-1 acts as a selective inhibitor of STAT3 activation, thereby modulating the STAT3 signaling cascade.
Automatically generated - may contain errors
Lab products found in correlation
Penicillin and streptomycin, by Thermo Fisher Scientific (1 mentions)
Prmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Thp 1, by American Type Culture Collection (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (1 mentions)
Thp 1, by Thermo Fisher Scientific (1 mentions)
Sorafenib, by MedChemExpress (1 mentions)
As1517499, by MedChemExpress (1 mentions)
Erastin, by MedChemExpress (1 mentions)
Zymosan tlrl zyn, by InvivoGen (1 mentions)
Hkca tlrl hkca, by InvivoGen (1 mentions)
Sis17, by MedChemExpress (1 mentions)
L nmma, by Beyotime (1 mentions)
Jsh 23, by MedChemExpress (1 mentions)
Trehalose 6 6 dibehenate, by InvivoGen (1 mentions)
3 protocols using stat3 in 1
1
Cell lines and chemical compounds
2
STAT3 Inhibition Impacts Cell Proliferation
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Cells were treated with DMEM supplemented with either 5 or 10 µM STAT3-IN-1 (MedChemExpress) in DMSO, or DMSO only, for 24 or 48 h, as described previously. Cell seeding was adjusted as necessary to compensate for the slightly diminished proliferative rate in the 10 µM STAT3-IN-1 treatments. Cells were seeded to permit continuous growth over the indicated time periods, without the need for media changes.
3
Immune Response Modulation Protocols
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Heat-killed C. albicans (HKCA; tlrl-hkca), zymosan (tlrl-zyn) and Trehalose-6,6-dibehenate (TDB; tlrl-tdb) were purchased from Invivogen. Mannan (M3640) were purchased from Sigma-Aldrich. The HDAC11 inhibitors FT895 (HY-112285) and SIS17 (HY-128918), SYK inhibitor R406 (HY-12067), NF-κB inhibitor JSH-23 (HY-13982) and STAT3 inhibitor STAT3-IN-1 (HY-100753) were purchased from MedChemExpress. iNOS inhibitors S-methylisothiourea hemisulfate salt (SMT; S0008) and L-NMMA (S0011) were purchased from Beyotime Biotechnology. The SYK inhibitor R406 (S2194) was purchased from Selleck. The antibodies used in this study shown in Supplementary Table 1 .
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