All procedures were performed at 45°C on a microscope stage heated with a thermoplate (TP-110R-100; Tokai Hit, Japan). The cell culture was poured into a tunnel chamber assembled by taping a coverslip (Nakane and Nishizaka, 2017 (link)), and both ends of the chamber were sealed with nail polish to keep from drying the sample. The position of the cell was visualized by infrared light from a halogen lamp with a bandpass filter (FBH850/40; Thorlabs) at a fluence rate of 1 μmol m−2 s−1. The cells were subjected to lateral light stimulus by an LED from the right side of the microscope stage at an angle of 5°. White LEDs at 20 and 500 μmol m−2 s−1 were used as moderate and strong light stimuli for phototaxis, respectively. Blue, teal, green, orange, red, and far-red light were applied by a monochromatic LED, M450LP1, M490L4, M530L3, M625L3, and M730L4 (Thorlabs), respectively. The LED light was collimated by the condenser lens and combined by dichroic mirrors (FF470-Di01, FF509-FDi01, FF560-FDi01, FF685-Di02; Semrock) to apply multicoloured light simultaneously. The wavelength of the resultant light was measured by a spectrometer (BIM-6002A, BroLight, China). Light intensity was measured with a power metre (Q82017A; Advantest, Japan).
Ff470 di01
Manufactured by IDEX Corporation
Sourced in United States
The FF470-Di01 is a laboratory equipment product. It is designed for specific technical applications. No further details can be provided without the risk of unintended interpretation or extrapolation.
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Lab products found in correlation
3 protocols using ff470 di01
1
Microscopic Phototaxis Assay at Optimal Temperature
2
High-Resolution Optical Microscopy Imaging
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
An inverted
optical microscope (Olympus, IX73, Japan) with an oil immersion objective
lens (Olympus, UPlan FLN ×100/1.3 NA, oil) was used to achieve
high-resolution excitation through a photomask. A laser at 355 nm
(PNV-M02510-1×0, Teem Photonics, France) and a laser diode with
a wavelength of 445 nm (TC20-4450-4.5, Neoark, Tokyo, Japan) were
used as excitation. The emission images were collected using an electron
multiplying (EM) CCD camera (iXon Ultra/life 897, Andor Technology,
UK). A dichroic mirror (FF470-Di01, Semrock, USA) and a long-pass
filter (LP02-473RU, Semrock, USA) were used to excite the sample and
collect only the sample emission. For the conditions of the CCD and
amplifier, appropriate values of gain and setting temperature were
selected depending on the purpose. The excitation power was measured
using a photodiode power sensor (S130VC, Thorlabs, Germany). All measurements
were performed in air at RT.
optical microscope (Olympus, IX73, Japan) with an oil immersion objective
lens (Olympus, UPlan FLN ×100/1.3 NA, oil) was used to achieve
high-resolution excitation through a photomask. A laser at 355 nm
(PNV-M02510-1×0, Teem Photonics, France) and a laser diode with
a wavelength of 445 nm (TC20-4450-4.5, Neoark, Tokyo, Japan) were
used as excitation. The emission images were collected using an electron
multiplying (EM) CCD camera (iXon Ultra/life 897, Andor Technology,
UK). A dichroic mirror (FF470-Di01, Semrock, USA) and a long-pass
filter (LP02-473RU, Semrock, USA) were used to excite the sample and
collect only the sample emission. For the conditions of the CCD and
amplifier, appropriate values of gain and setting temperature were
selected depending on the purpose. The excitation power was measured
using a photodiode power sensor (S130VC, Thorlabs, Germany). All measurements
were performed in air at RT.
3
Phototaxis Analysis of Motile Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All procedures were performed at 45 °C on a microscope stage heated with a thermoplate (TP-110R-100; Tokai Hit, Japan). The cell culture was poured into a tunnel chamber assembled by taping a coverslip (7) (link), and both ends of the chamber were sealed with nail polish to keep from drying the sample. The position of the cell was visualized by infrared light from a halogen lamp with a bandpass filter (FBH850/40; Thorlabs) at a fluence rate of 1 μmol m -2 s -1 . The cells were subjected to lateral light stimulus by an LED from the right side of the microscope stage at an angle of 5 degrees. White LEDs at 20 and 500 μmol m -2 s -1 were used as moderate and strong light stimuli for phototaxis, respectively. Blue, teal, green, orange, red, and far-red light were applied by a monochromatic LED, M450LP1, M490L4, M530L3, M625L3, and M730L4 (Thorlabs), respectively. The LED light was collimated by the condenser lens and combined by dichroic mirrors (FF470-Di01, FF509-FDi01, FF560-FDi01, FF685-Di02; Semrock) to apply multicoloured light simultaneously. The wavelength of the resultant light was measured by a spectrometer (BIM-6002A, BroLight, China). Light intensity was measured with a power metre (Q82017A; Advantest, Japan).
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