ALA (Cat# SML0415-25MG), (S)-(+)-α-Tetralol (Cat# 87649), and β-NADH phosphate disodium salt (Cat# 50020) were purchased from Sigma-Aldrich (MO, United States). Fresh ALA was dissolved in DMSO for each cell experiment (10 mM stock solution). Primary antibodies against GAPDH (Cat# ab181602), p-STAT3 (Ser727, Cat# ab32143), STAT3 (Cat# ab109085), and AKR1C1 (Cat# ab192785) were purchased from Abcam (Boston, MA, United States). The TMT10plex Isobaric Mass Tag Labelling Reagents (Cat# 90113) were obtained from Thermo Fisher Scientific (Boston, MA, United States). The Dual Luciferase Reporter Gene Assay Kit (Cat# RG028) was purchased from Beyotime (Shanghai, China). Cell Counting Kit 8 (CCK-8, Cat# ab228554) was obtained from Abcam (Boston, MA, United States).
P stat3 ser727
Manufactured by Abcam
Sourced in United States
P-STAT3 (Ser727) is a phospho-specific antibody that recognizes the serine 727 phosphorylation site of STAT3, a transcription factor involved in various cellular processes. This antibody can be used to detect and quantify the phosphorylation of STAT3 at serine 727 in a range of applications, such as Western blotting, immunoprecipitation, and flow cytometry.
Automatically generated - may contain errors
Lab products found in correlation
P stat3 tyr705, by Abcam (2 mentions)
Akr1c1, by Abcam (1 mentions)
Cell counting kit 8 cck 8, by Abcam (1 mentions)
Gapdh, by Abcam (1 mentions)
Stat3, by Abcam (1 mentions)
Dual luciferase reporter gene assay kit, by Beyotime (1 mentions)
Photomicroscope, by Olympus (1 mentions)
3 3 diaminobenzidine chromogen, by Thermo Fisher Scientific (1 mentions)
P ampk, by Abcam (1 mentions)
Il 17, by Abcam (1 mentions)
Il 1β, by Abcam (1 mentions)
Interleukin 6 (il 6), by Abcam (1 mentions)
Lysis buffer, by Merck Group (1 mentions)
P akt thr308, by Cell Signaling Technology (1 mentions)
P akt ser473, by Cell Signaling Technology (1 mentions)
Anti mouse igg secondary antibody, by Merck Group (1 mentions)
Total stat3, by Abcam (1 mentions)
Claudin 1, by Cell Signaling Technology (1 mentions)
Snail, by Cell Signaling Technology (1 mentions)
Total akt, by Cell Signaling Technology (1 mentions)
3 protocols using p stat3 ser727
1
Protein Expression and Signaling Assays
2
Molecular Pathway Analysis in Joint Tissues
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Joint tissues were incubated overnight at 4 °C with primary antibodies against SMILE (Abcam, Cambridge, UK), B-cell activating factor receptor (BAFF-R) (Abcam), pAMPK (Abcam), mTOR (Cell Signaling, MA, USA), pSTAT3 ser727 (Abcam), Pstat3 Tyr705 (Abcam), IL-1β (Abcam), IL-6 (Abcam), IL-17(Abcam). Subsequently, samples were incubated with a biotinylated streptavidin–peroxidase complex for 1 h, and the signals were developed using chromogen 3,3′- diaminobenzidine (Thermo Scientific, Rockford, IL, USA). The sections were examined under a photomicroscope (Olympus, Tokyo, Japan). The number of positive cells in high-power digital images (magnification, ×400) was counted using Adobe Photoshop software (Adobe, San Jose, CA, USA). Stained cells were counted independently by three observers, and the mean values were evaluated.
3
Protein Extraction and Western Blot Analysis
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Whole-cell protein was isolated as described previously [7] . Briefly, for tissue protein extraction, the tumor tissues were homogenized in ice-cold RIPA (radioimmunoprecipitation assay) lysis buffer (Millipore). The tissue lysates were incubated at 4°C for 1 hour with rotation followed by clarification of tissue debris by centrifugation at 12, 000 rpm for 10 minutes. The protein concentration of tumor extracts was determined using the BCA Protein Assay Kit (Pierce). Western blotting was performed as previously described [7] . Briefly, The following antibodies to E-cadherin (Cell Signaling), vimentin (Cell Signaling), snail (Cell Signaling), slug (Cell Signaling), ZO-1 (Cell Signaling), N-cadherin (Cell Signaling), claudin-1 (Cell Signaling), β-actin (Sigma Aldrich), total STAT3 (Abcam), pSTAT3 (Ser727) (Abcam), pSTAT3 (Tyr705) (Abcam), pSTAT (Tyr694) (Cell Signaling), total AKT (Cell Signaling), pAKT (Ser473) (Cell Signaling), pAKT (Thr308) (Cell Signaling), and pERK (Cell Signaling) were applied for protein detection. Antibody binding was revealed using an HRPconjugated anti-rabbit IgG or anti-mouse IgG secondary antibody (Sigma). Antibody complexes were detected using Immobilon Western Chemiluminescent HRP Substrate (Millopore) and exposure to Tanon 6200 Luminescent Imaging Workstation (Tanon Science & Technology Co., Ltd., Shanghai, China).
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