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Pluronic f127 f127

Manufactured by BASF
Sourced in Germany, United States

Pluronic F127 (F127) is a non-ionic triblock copolymer consisting of polyethylene oxide (PEO) and polypropylene oxide (PPO) units. It is a white, waxy solid at room temperature and is soluble in water and organic solvents. F127 is commonly used as a surfactant, emulsifier, and dispersant in various scientific and industrial applications.

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5 protocols using pluronic f127 f127

1

Pluronic F127 Nanocarrier Protocol

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Pluronic® F127 (F127) was supplied by BASF Corporation (Ludwigshafen, Germany) without further purification. D-α-tocopheryl polyethylene glycol 1000 succinate (Vitamin E TPGS or TPGS) was purchased from Eastman Chemical Company (Kingsport, TN, USA). Rhodamine123 (Rho123) were purchased from Sigma-Aldrich Co. (StLouis, MO, USA). 1,10-Dioctadecyl-3,3,30,30- tetramethylindo-tricarbocyanine iodide (DiR), a near-infrared dye, was obtained from Biotium (Invitrogen, USA). β-galactosidase (β-Gal) was purchased from Meilune company (Dalian, China). Dulbecco’s Modified Eagle’s Medium (high glucose) (DMEM), fetal bovine serum (FBS), trypsine-EDTA (0.25%) and penicillin - streptomycin were purchased from Gibco (CA, USA). Cells counting kit-8 (CCK-8) were purchase from DOJINDO laboratories (Shanghai, China).
High performance liquid chromatography (HPLC) grade methanol were purchased from Merck KgaA (Darmstadt, Germany). All other solvents were analytical grade.
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2

Pluronic F127-Based Antioxidant Delivery System

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Pluronic F127 (F127, molecular weight =12,600) was purchased from BASF SE (Ludwigshafen, Germany). Tocopherol and 3,3′-dithiodipropionic acid were purchased from Aladdin Industrial Corporation (Shanghai, People’s Republic of China). Dicyclohexylcarbodiimide (DCC) and 4-dimethylam-inopyridine (DMAP) were obtained from GL Biochem., Ltd. (Shanghai, People’s Republic of China). Millipore water was prepared with a Milli-Q Plus System (Merck Millipore Co., Billerica, MA, USA). All chemicals were in analytical grade.
Human hepatoma cell line Bel 7402 and human hepatic cell line L02 were supplied by American Type Culture Collection (ATCC, Rockville, MD, USA). Dulbecco’s Modified Eagle’s Medium, fetal bovine serum, phosphate-buffered saline, penicillin–streptomycin, and 0.25% (w/v) trypsin/1 mM EDTA were obtained from Thermo Fisher Scientific (Waltham, MA, USA).
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3

Nanoparticle-based Anticancer Drug Delivery

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PTX was a gift of Samyang Biopharm (Seoul, Korea). Pluronic F127 (F127) was a gift from BASF (New York, NY, USA). Abraxane was obtained from Celgene Corporation (Summit, NJ, USA). Human serum albumin (≥96% agarose gel electrophoresis) and hexadecyltrimethylammonium bromide (CTAB) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Reagents for sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) were purchased from Bio-Rad (Hercules, CA, USA). Terminal deoxynucleotidyl transferase dUTP nick end labeling kit (DeadEnd Fluorometric TUNEL System) was purchased from Promega (Madison, WI, USA). All other reagents were purchased from Sigma-Aldrich (St. Louis, MO, USA).
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4

Formulation and Characterization of CFZ

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CFZ was purchased from Shenzhen Chemical Co. Ltd. (Shanghai, China). Pluronic F127 (F127) was a gift from BASF (New York, NY, USA). Human serum albumin (HSA; ≥ 96% purity assessed by agarose gel electrophoresis, Cat. No. A1653) and (2-hydroxypropyl)-β-cyclodextrin were from Sigma-Aldrich (St. Louis, MO, USA). All other reagents were from Thermo Fisher Scientific (Waltham, MA, USA).
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5

Curcumin-Pluronic Hydrogel for Uterine Repair

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Curcumin (purity higher than 98%) and DMEM medium were purchased from Shanghai Adamas Reagent Co., Ltd Pluronic®F127 (F127) and Pluronic®F68 (F68) were purchased from BASF SE (Germany). Growth factor-reduced Matrigel was purchased from Corning Incorporated (USA). Recombinant Bovine bFGF, Trizol and BCA Protein Assay Kit were purchased from Beyotime Biotechnology. hESC and HUVEC cell lines were purchased from ATCC. Thiazolyl Blue (MTT, purity higher than 98%) was purchased from Biofroxx (Germany), DMSO was purchased from Aladdin Reagent Shanghai Co., Ltd and Endothelial Cell Medium (ECM) was purchased from ScienCell (USA). Fetal bovine serum (FBS) was purchased from Zhejiang Tianhang Biotechnology Co., Ltd anti-TGF-β1 (21898-1-AP, Proteintech, Rosemont, IL, USA). Cyanine7 dye was synthesized in our laboratory. Cross-linked sodium hyaluronate gel for the uterine cavity (HA-gel) was purchased from Changzhou Bioregen Biomedicine Co., Ltd.
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