Spinner flasks

The cell lines employed in this study were obtained from the American Type Culture Collection (ATCC, USA), including 293T cells (human embryonic kidney cells), HCC1937 cells, MDA-MB-231 cells (human triple negative-breast carcinoma), and 4T1 cells (mouse mammary carcinoma cells). These cell lines were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Gibco-Thermo Fisher Scientific, USA) supplemented with 10% fetal bovine serum (FBS, Gibco). Specifically, 293T cells were cultured in suspension using a serum-free medium (Basalmedia, Shanghai, China) in spinner flasks (Jetbiofil, Guangzhou, China). To generate the 4T1^ApoA1 and HCC1937^ApoA1 cells, lentiviral transduction was employed followed by subsequent puromycin selection. All cell lines were incubated at 37 °C in a 5% CO₂ atmosphere.

The cell lines used in this study included Raji (Human Burkitt’s lymphoma cell), Farage (Human B-cell lymphoma cell), Pffeifer (Diffuse large B-cell lymphoma cell), Nalm-6 (Human B-cell lymphocyte leukemia cell), Jurkat (Human T-cell lymphocyte leukemia cell), CEM (Human T-cell lymphocyte leukemia cell), HEK293 (human embryonic kidney cell), and Hela-S3 (Human cervix carcinoma cell), K562 (Human chronic myelogenous leukemia cell), and K562/CD19 (K562 cells transfected with human CD19 antigen). HEK293 and Hela-S3 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM; Cat# 11965092, Gibco-Thermo Fisher Scientific, USA) supplemented with 10% fetal bovine serum (FBS; Cat#16000044, Gibco). Raji, Farage, Pffeifer, Nalm-6, Jurkat, CEM, K562, and K562/CD19 cells were cultured in RPMI 1640 medium (Cat# 11875093, Gibco) supplemented with 10% FBS (Cat#16000044, Gibco). Hela-S3 cells were cultured in suspension in a serum-free medium (Cat# H740KJ, Basalmedia, Shanghai, China) in spinner flasks (Cat# TCB002002, Jetbiofil, Guangzhou, China). All cells were incubated at 37 °C with 5% CO₂ atmosphere.

The cell lines used in this study included HEK293 (human embryonic kidney cell), 4T1 (mouse mammary carcinoma cell), CT26 (mouse colon carcinoma cell), MC38 (mouse colon carcinoma cell), H22 (mouse hepatocellular carcinoma cell), and Hela-S3 (Human cervix carcinoma cell). HEK293 (Cat# CRL-1573, RRID: CVCL_0045), 4T1 (Cat# CRL-2539, RRID: CVCL_0125), CT26 (Cat# CRL-2638, RRID: CVCL_7256), and Hela-S3 (Cat# CCL-2.2, RRID: CVCL_0058) cells were obtained from the American Type Culture Collection (ATCC; USA). MC38 cells (RRID: CVCL_B288) were obtained from the National Cancer Institute (NCI; USA). H22 cells (ID: 3111C0001CCC000309) were obtained from the China Center for Type Culture Collection (CCTCC; Wuhan, China; http://cellresource.cn). HEK293, 4T1, CT26, MC38, and Hela-S3 cells were cultured in Dulbecco's modified Eagle's medium (DMEM; Cat# 11965092, Gibco-Thermo Fisher Scientific, USA) supplemented with 10% fetal bovine serum (FBS; Cat#16000044, Gibco). H22 cells were cultured in RPMI 1640 medium (Cat# 11875093, Gibco) supplemented with 10% FBS (Cat#16000044, Gibco). Hela-S3 cells were cultured in suspension in serum-free medium (Cat# H740KJ, Basalmedia, Shanghai, China) in spinner flasks (Cat# TCB002002, Jetbiofil, Guangzhou, China). All cells were incubated at 37 °C with 5% CO₂ atmosphere.