Rabbit anti pmlc thr18 ser19

Manufactured by Cell Signaling Technology

Sourced in United States

Rabbit anti-pMLC (Thr18/Ser19) is a primary antibody that specifically recognizes phosphorylated myosin light chain at threonine 18 and serine 19 residues.

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Lab products found in correlation

Rhodamine phalloidin, by Thermo Fisher Scientific (6 mentions) Goat anti mouse cy3, by Jackson ImmunoResearch (2 mentions) Alexa fluor 488 goat anti rabbit, by Thermo Fisher Scientific (2 mentions) Dasatinib, by Santa Cruz Biotechnology (2 mentions) Alexa fluor 594 goat anti rabbit, by Thermo Fisher Scientific (2 mentions) Rabbit anti nmhc2a, by Merck Group (2 mentions) Rabbit anti vinculin, by Thermo Fisher Scientific (2 mentions) Mouse anti β actin, by Merck Group (2 mentions) 4 6 diamidino 2 phenylindole dihydrochloride dapi, by Merck Group (2 mentions) Mouse anti α tubulin, by Merck Group (1 mentions) Rabbit anti calnexin, by Merck Group (1 mentions) Rabbit anti src, by Santa Cruz Biotechnology (1 mentions) Mouse anti phospho tyrosine, by Merck Group (1 mentions) Hanks balanced salt solution (hbss), by Lonza (1 mentions)

Spelling variants of this product

Anti-pMLC (16 citations) Rabbit anti-pMLC (5 citations)

2 protocols using rabbit anti pmlc thr18 ser19

Dasatinib-based immunofluorescence and immunoblotting

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Dasatinib (Santa Cruz Biotechnology, Dallas, TX, USA) was used at 300 nM in complete medium for 1 h before the experiment. The following antibodies were used at 1/200 for immunofluorescence microscopy (IF) or 1/1000 for immunoblotting (IB): mouse anti-β-actin (#A1978, Sigma, St Louis, MO, USA); rabbit anti-NMHC2A (#M8064, Sigma, St Louis, MO, USA); rabbit anti-calnexin (#AB2301, Merck Millipore, Burlington, MA, USA), rabbit anti-vinculin (#700062, Fisher Scientific, Waltham, MA, USA); rabbit anti-pMLC (Thr18/Ser19) (#3674, Cell Signaling, Danvers, MA, USA); and monoclonal anti-Src (GD11, abcam, Cambridge, UK). For IF analysis, DNA was stained with 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI; Sigma, St Louis, MO, USA) and actin with Rhodamine Phalloidin (Thermo Fisher Scientific, Waltham, MA, USA) at 1/200. Secondary antibodies were used at 1/500: goat anti-rabbit Alexa Fluor 488 (Invitrogen, Waltham, MA, USA), goat anti-rabbit Alexa Fluor 594 (Invitrogen, Waltham, MA, USA), and goat anti-mouse Cy3 (Jackson ImmunoResearch, West Grove, PA, USA). For IB, goat anti-rabbit or anti-mouse HRP (Abliance, Compiègne, France) was used at 1/10,000.

Brito C., Pereira J.M., Mesquita F.S., Cabanes D, & Sousa S. (2023). Src-Dependent NM2A Tyrosine Phosphorylation Regulates Actomyosin Remodeling. Cells, 12(14), 1871.

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Dasatinib Modulates Intoxication Responses

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Dasatinib (Santa Cruz Biotechnology) was used at 300 nM in complete medium for 1 hour prior to LLO intoxication. LLO was expressed in E. coli BL21(DE3) and purified as described in [84] .
Intoxications and washes were carried in Hanks' balanced salt solution (HBSS, Lonza). The following antibodies were used at 1/200 for immunofluorescence microscopy (IF) or 1/1,000 for immunoblotting (IB): rabbit anti-Src pTyr 419 (#44-660G, Invitrogen), mouse anti-active Src (#AHO0051, Invitrogen), rabbit anti-Src (#sc-18, Santa Cruz), mouse anti-β-actin (#A1978, Sigma); mouse anti-phosphotyrosine (#05-321, Millipore); rabbit anti-NMHC2A (#M8064, Sigma); rabbit anti-calnexin (#AB2301, Millipore), rabbit anti-vinculin (#700062, Fisher Scientific), rabbit anti-pMLC (Thr18/Ser19) (#3674, Cell Signaling), rabbit anti-NMY-2 (against residues 945-1368), mouse anti-α-tubulin (#T5168, Sigma). For IF analysis, DNA was stained with 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI; Sigma) and actin with Rhodamine Phalloidin (Thermo Fisher Scientific) at 1/200; secondary antibodies were used at 1/500: goat anti-rabbit Alexa Fluor 488 (Invitrogen), goat anti-rabbit Alexa Fluor 594 (Invitrogen), goat anti-mouse Cy3 (Jackson ImmunoResearch). For IB, goat anti-rabbit or anti-mouse HRP (PARIS) were used at 1/10,000.

Brito C., Mesquita F.S., Osório D.S., Pereira J., Billington N., Sellers J.R., Carvalho A.X., Cabanes D., & Sousa S. (2020). Src-dependent NM2A tyrosine-phosphorylation regulates actomyosin dynamics.

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