Notch1, Notch2 (Mitsiadis et al., 1995 (link), 1998 (link)), Adam10 (a gift from Prof. Yufang Zheng), Ameloblastin (Ambn; a gift from Dr Yoshihiko Yamada) riboprobes were used for in situ hybridization on 14 μm cryosections, as previously described (Mitsiadis et al., 1998 (link)). The hybridization signal was detected using the NBT/BCIP substrate solution, sections were mounted with Glicergel® mounting medium (Dako) and imaged with Leica DM6000 microscope equipped with the Leica DFC420C camera. Images were processed with the Leica Application Suite (LAS) software.
Glicergel mounting medium
Manufactured by Agilent Technologies
Glicergel® is a mounting medium for microscopy specimens. It is a clear, viscous solution that is used to mount and preserve samples for observation under a microscope. The product provides a stable, transparent environment to hold and protect the specimen.
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2 protocols using glicergel mounting medium
1
In Situ Hybridization of Dental Markers
2
ALP Activity Assessment in Tissue
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ALP activity was assessed in paraffin sections. Briefly, sections were deparaffinized, rehydrated, and incubated in 1% MgCl2 (Sigma-Aldrich, M8266) in Tris-Maleate buffer overnight at 4°C, followed by the addition of NBT/BCIP substrates (Sigma-Aldrich). Sections were finally mounted with Glicergel® mounting medium (Dako) and analyzed using a Leica DM6000 microscope.
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