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Casp3 9661

Manufactured by Cell Signaling Technology
Sourced in United States

Casp3 (9661) is a primary antibody product offered by Cell Signaling Technology. It is used to detect the expression of Caspase-3, a key enzyme involved in the execution phase of cell apoptosis.

Automatically generated - may contain errors

2 protocols using casp3 9661

1

Immunoblotting Antibodies for Protein Analysis

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Immunoblotting was performed62 (link) using antibodies against: ATF4 (11815), phospho-eIF2 (3398), total eIF2 (9722), and Casp3 (9661) all from Cell Signaling Technology (Danvers, MA, USA); Flag-M2 (F1804) from Sigma-Aldrich (St Louis, MO, USA); Nupr1 (ab6028) from Abcam (Cambridge, MA, USA); Bex2 (sc48966), ATF3 (sc188), GADD34 (sc825), CHOP (sc7351), and ERK2 (sc154) were all from Santa Cruz Biotechnology (Dallas, TX, USA).
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2

Characterization of Cellular Markers

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For flow cytommetry, CD31 (BD558737), CD45 (B553078), LY76/TER119 (BD553672), and CD24-PE (BD553262) antibodies were from BD Biosciences, CD61-APC (MCD6105) from Invitrogen, and CD29-FITC (MCA2298FB) from Serotech were used. For immunohistology, KRT6 (PRB-169P) from Covance, KRT17 (ab53707), TNF/TNFα (ab8871), F4/80 (ab6640), and 8-O-dG (ab48508) from Abcam, KRT14 (gift from Dr Segre), PGR (gift from Dr Clarke), CASP3 (9661) from Cell Signaling Technology, MKI67 (NCL-L-Ki67-MM1) from Leica, ESR1/ERα (sc-542) and RELA/p65 (sc-8008) from Santa Cruz Biotechnology, TNFRSF11A/TNR11 (AF692) and TNFSF11/TNF11 (AF462) from R&D Systems, SQSTM1 (BML-PW9860) from Enzo, LC3 (NB100-2331) and IL1B/IL-1β (NBP1-19775) from Novus Biologics, and KRT8 (TROMA-1) and BrdU (G3G4) from the Developmental Studies Hybridoma Bank were used.
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