S8007

Manufactured by Selleck Chemicals

S8007 is a laboratory equipment designed for measuring and recording temperature. It features a digital display and precision temperature sensors, enabling accurate temperature monitoring in various scientific and research applications.

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Lab products found in correlation

S1060, by Selleck Chemicals (2 mentions) Olaparib, by Selleck Chemicals (2 mentions) Envision 2103, by PerkinElmer (1 mentions) Celltiter glo luminescent cell viability assay, by Promega (1 mentions) Camptothecin, by Selleck Chemicals (1 mentions) Flavopiridol, by Selleck Chemicals (1 mentions) Aphidicolin, by Merck Group (1 mentions) Doxycycline, by Merck Group (1 mentions) H8627, by Merck Group (1 mentions) Roscovitine, by Selleck Chemicals (1 mentions) Hydroxyurea, by Merck Group (1 mentions) A0781, by Merck Group (1 mentions) D1916, by Merck Group (1 mentions) Ve 821, by Selleck Chemicals (1 mentions) S1230, by Selleck Chemicals (1 mentions) Azd5438, by Selleck Chemicals (1 mentions)

2 protocols using s8007

PrimPol Knockdown Cell Viability Assay

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Cells were seeded in 96-well flat-bottom plates at a density of 2000 cells/well following 24 h of PrimPol siRNA knockdown. Stock solutions of each drug were prepared in sterile water or dimethyl sulfoxide (DMSO) as appropriate and further diluted in growth medium. Cells were allowed to grow for 6 days in drug containing medium (VE-821, SelleckChem S8007, Olaparib, SelleckChem S1060) and cell viability was measured with the CellTiter-Glo Luminescent Cell Viability Assay (Promega G7572) following manufacturer’s instructions. Luminescence was then measured with a PerkinElmer Envision 2103 multilabel plate reader. Viability was calculated as the luminescence signal ratio of treated versus untreated samples. Analysis and statistical test were performed using Microsoft Excel and GraphPad Prism v9.

Martínez-Torres A, & Miledi R. (2001). Expression of γ-aminobutyric acid ρ1 and ρ1Δ450 as gene fusions with the green fluorescent protein. Proceedings of the National Academy of Sciences of the United States of America, 98(4), 1947-1951.

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DNA Damage and Replication Stress Compounds

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Unless stated otherwise the following compounds were used in this manuscript at the indicated final concentrations: ATRi Az-20 (1 μM, 5198, Tocris), ATRi VE-821 (10 μM, S8007, Selleckchem), Camptothecin (50 nM, S1288, Selleckchem), Roscovitine (20 μM, S1153, Selleckchem), Olaparib (10 μl, S1060, Selleckchem), DRB (100 μM, D1916, Sigma), Flavopiridol (1.25 μM, S1230, Selleckchem), AZD5438 (1 μM, S2621, Selleckchem), Hydroxyurea (HU, 2 mM, H8627, Sigma), Doxycycline (1-1000 ng/mL, D9891, Sigma), CDC7i (25 μM, S2742, Selleckchem), Aphidicolin (0.2 μM, A0781, Sigma), Diospyrin D (10 μM, gift of Dr. Pavel Janscak).

Teloni F., Michelena J., Lezaja A., Kilic S., Ambrosi C., Menon S., Dobrovolna J., Imhof R., Janscak P., Baubec T, & Altmeyer M. (2019). Efficient Pre-mRNA Cleavage Prevents Replication-Stress-Associated Genome Instability. Molecular Cell, 73(4), 670-683.e12.

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