Mir 141 3p mimic

The miR-141-3p siRNA, miR-141-3p mimic, and their corresponding controls were purchased from Shanghai GenePharma Co., Ltd. Cells (2×10⁵ cells/ml) were transfected with Lipofectamine^® 2000 (Invitrogen; Thermo Fisher Scientific, Inc.) and divided into the following groups: i) NG; ii) HG; iii) HG + MALAT1 overexpression; iv) HG + miR-141-3p siRNA scramble control (NC3), cells were transfected with 10 nM miR-141-3p siRNA scramble control (5′-ACGUGACACGUUCGGAGAATT-3′) at 37°C for 24 h, according to previous study (21 (link)), then cultured in HG medium for 24 h; v) HG + miR-141-3p siRNA (si-miR), cells were transfected with 10 nM miR-141-3p siRNA (5′-CCAUCUUUACCAGACAGUGUUA-3′) at 37°C for 24 h, then cultured in HG medium for 24 h; vi) HG + MALAT1 overexpression + miR-141-3p overexpression scramble control (MALAT1 + NC4), cells were transfected with 10 nM miR-141-3p mimic scramble control (5′-ACGUGACACGUUCGGAGAATT-3′) and 50 nM pcDNA3.1-MALAT1 at 37°C for 24 h, then cultured in HG medium for 24 h; and vii) HG + MALAT1 overexpression + miR-141-3p overexpression (MALAT1 + miR), cells were transfected with 10 nM miR-141-3p mimic (5′-UAACACUGUCUGGUAAAGAUGG-3′) and 50 nM pcDNA3.1-MALAT1 at 37°C for 24 h, then cultured in HG medium for 24 h. Transfection efficiency was determined via RT-qPCR.