Soil respiration rates were measured with a field-portable infrared gas analyzer (IRGA; LiCor 8100-A, Lincoln, Nebraska, USA) in PVC collars, randomly placed within each plot. There were 2 collars per plot permanently inserted into the soil (5 cm deep) 1 day before treatments started, and collars were open to rainfall and litterfall, except during measurements (Taneva and Gonzalez-Meler, 2011 (link)). The chosen measurement times were 12:00 h (11:00–13:00 h) and 18:00 h for all plots (18:00–20:00 h). Measurements were carried out at the beginning and at the end of the 30-day warming period for three consecutive days. Soil temperature and moisture were continuously monitored using the sensors Theta Probe ML2x for moisture Theta Probe ST2 for temperature coupled to a DL2 datalogger (Delta-T Devices, UK).
Licor 8100 a
Manufactured by LI COR
Sourced in United States
The LI-COR 8100-A is a soil gas flux system designed for measuring soil respiration rates. It utilizes an automated, closed-chamber method to capture and analyze the changes in carbon dioxide (CO2) concentration within a chamber placed on the soil surface. The system provides accurate and reliable measurements of soil CO2 efflux, a key parameter for understanding soil carbon dynamics and ecosystem processes.
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Lab products found in correlation
2 protocols using licor 8100 a
1
Soil Respiration Measurement Protocol
2
In Situ CO2 Flux Measurement
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Monthly in situ CO2 flux measurements beginning March and ending September 2017 were taken using a LICOR-8100A (LICOR Inc., Lincoln, Nebraska, USA) portable infrared gas analyser and custom Perspex chambers of 24 cm diameter and 30 cm height. Small 9V batteryoperated fans were installed within the chambers to circulate the air. The two chambers, one clear and one covered with a blackout cloth, were sealed to the collars using rubber mastic (Terostat), and consecutive measurements were taken with a brief aeration period as the chambers were exchanged. Each measurement period was five minutes, with a 20 second premeasurement stabilisation period. Chamber flux measurements were usually taken between 8 am and 2 pm, although this was sometimes altered due to weather conditions. Each collar was measured once with a clear-chamber and once with a blackout chamber on each visit except when adverse weather conditions prevented a full dataset being collected.
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