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Adipogenic differentiation medium

Manufactured by Takara Bio
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Sourced in Japan

Adipogenic differentiation medium is a specialized culture medium designed to promote the differentiation of cells into adipocytes (fat cells). It contains a specific combination of growth factors, hormones, and other nutrients that support the conversion of precursor cells into mature adipocytes.

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Lab products found in correlation

Osteogenic differentiation medium, by Merck Group (2 mentions) Oil red o, by Merck Group (2 mentions) Alizarin red s, by Fujifilm (2 mentions)

2 protocols using adipogenic differentiation medium

1

Adipogenic and Osteogenic Differentiation

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To induce adipogenic or osteogenic differentiation, MSCs were cultured in adipogenic differentiation medium (Takara Bio, Shiga, Japan) or osteogenic differentiation medium (Sigma-Aldrich), respectively, for 14 days following the manufacturers’ protocols. Oil Red O (Sigma-Aldrich) and Alizarin Red S (FUJIFILM Wako Pure Chemical, Osaka, Japan) were used to analyze adipogenic and osteogenic differentiation, respectively.
Ishiuchi N., Nakashima A., Doi S., Kanai R., Maeda S., Takahashi S., Nagao M, & Masaki T. (2021). Serum-free medium and hypoxic preconditioning synergistically enhance the therapeutic effects of mesenchymal stem cells on experimental renal fibrosis. Stem Cell Research & Therapy, 12, 472.
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2

Adipogenic and Osteogenic Differentiation Assay

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ASCs were cultured in adipogenic differentiation medium (Takara Bio) or osteogenic differentiation medium (Sigma-Aldrich) for 14 days in accordance with the manufacturers’ protocols. Oil Red O (Sigma-Aldrich) and Alizarin Red S (FUJIFILM Wako Pure Chemical, Osaka, Japan) were used to assess adipogenic and osteogenic differentiation, respectively. The stained area was estimated using ImageJ software by examining five randomly selected fields.
Ishiuchi N., Nakashima A., Maeda S., Miura Y., Miyasako K., Sasaki K., Uchiki T., Sasaki A., Nagamatsu S., Nakao N., Nagao M, & Masaki T. (2023). Comparison of therapeutic effects of mesenchymal stem cells derived from superficial and deep subcutaneous adipose tissues. Stem Cell Research & Therapy, 14, 121.
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