Caliper labchip gx system

Manufactured by PerkinElmer

Sourced in United States

The Caliper LabChip GX system is a microfluidic-based platform designed for automated electrophoretic analysis of DNA, RNA, and protein samples. The system employs advanced microfluidic technology to perform rapid and sensitive analyses, providing reliable results with minimal sample consumption.

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Lab products found in correlation

E220 system, by Covaris (2 mentions) Qrt pcr, by Roche (1 mentions) Idt xgen exome research panel v1, by Integrated DNA Technologies (1 mentions) Novaseq 6000, by Illumina (1 mentions) Nucleospin tissue kit, by Macherey-Nagel (1 mentions) Bioanalyzer gel, by Agilent Technologies (1 mentions) Sureselect xt methyl seq kit, by Agilent Technologies (1 mentions) Ez dna methylation gold kit, by Zymo Research (1 mentions) Geneamp pcr system 9700, by Thermo Fisher Scientific (1 mentions) Sequence scanner software v2, by Thermo Fisher Scientific (1 mentions) Abi 3730, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Caliper LabChip GX LabChip GX system LabChip GX LabChip system

3 protocols using caliper labchip gx system

Exome Sequencing of TN4 Family

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Genomic DNA was extracted from whole blood of 11 cases and two unaffected siblings (a brother and a sister) of TN4 (TN1-10 and TN12) with informed consent using NucleoSpin Tissue kit (#740952, Machery-Nagel, Duren, Germany) according to the manufacturer’s instructions. Exome library preparation and sequencing were performed by the Yale Center for Genome Analysis. Briefly, 1 µg of genomic DNA was sheared into fragments with the Covaris E220 system (Covaris, Woburn, MA, United States), followed by end-repair, A-tailing, ligation of multiplexing adaptors, and pre-capture ligation-mediated PCR amplification. The quantification and insert size distribution of product were determined using Caliper LabChip GX system (PerkinElmer Inc., Santa Clara, CA, USA). Exome library was captured using IDT xGen Exome Research Panel V1.0 (Integrated DNA Technologies, Coralville, IA, USA), and quantified by qRT-PCR using a commercially available kit (KAPA Biosystems, Wilmington MA, USA). Libraries were then loaded onto S4 flowcell and sequenced on the Illumina NovaSeq6000 platform using 101 bp paired-end sequencing reads according to Illumina protocols (Illumina Inc., San Diego, CA, USA).

Di Stefano G., Yuan J.H., Cruccu G., Waxman S.G., Dib-Hajj S.D, & Truini A. (2020). Familial trigeminal neuralgia – a systematic clinical study with a genomic screen of the neuronal electrogenisome. Cephalalgia, 40(8), 767-777.

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Whole Genome Bisulfite Sequencing

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Genomic DNA quality was determined by estimating the A260/A280 and A260/A230 ratios by nanodrop and concentration by Qubit dsDNA BR Assay. DNA integrity and size was confirmed by running an Agilent Bioanalyzer gel. Genomic DNA (1 µg) was sheared to a fragment length of 150–200 bp using a focused acoustic energy Covaris E220 system (Covaris, part#500003). Fragmented sample size distribution was determined by the Caliper LabChip GX system (PerkinElmer, Part#122000). Unmethylated C residues of enriched DNA were modified by bisulfite conversion using EZ DNA Methylation-Gold Kit (Zymo Research, part#D5005). Indexed paired-end whole genome sequencing libraries are prepared using SureSelect XT Methyl-Seq kit (Agilent, part#G9651B).

Pramio D.T., Vieceli F.M., Varella-Branco E., Goes C.P., Kobayashi G.S., da Silva Pelegrina D.V., de Moraes B.C., Allam A.E., De Kumar B., Jara G., Farfel J.M., Bennett D.A., Kundu S., Viapiano M.S., Rego Reis E.M., de Oliveira P.S., Passos-Bueno M.R., Rothlin C.V., Ghosh S, & Schechtman D. (2023). DNA methylation of the promoter region at the CREB1 binding site is a mechanism for the epigenetic regulation of brain specific PKMζ. Biochimica et biophysica acta. Gene regulatory mechanisms, 1866(1), 194909.

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DNA Amplification and Sanger Sequencing

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Primers were designed using Primer3 Plus software [17] . DNA was amplified on a GeneAmp ® PCR System 9700 (Thermo Fisher, MA, USA).
We checked the quantity and quality of PCR products, including product size and off-target amplification, using the Caliper ® LabChip GX System and its related software (PerkinElmer, MA, USA) according to the manufacturer's protocol. Sanger sequencing was then done at the labs of Eurofins Genomics (Germany) using the Big Dye Chemistry in an ABI3730 automated sequencer (Applied Biosystems, Thermo Fisher Scientific, USA) using the procedures recommended by the manufacturer on the PCR product. Sequencing files (ABI format) were then visualized and analyzed using Sequence Scanner Software ® v2.0 (Thermo Fisher Scientific, USA).

Yahia A., Hamed A.A.A., Mohamed I.N., Elseed M.A., Salih M.A., El-Sadig S.M., Siddig H.E., Nasreldien A.E.M., Abdullah M.A., Elzubair M., Omer F.Y., Bakhiet A.M., Abubaker R., Abozar F., Adil R., Emad S., Musallam M.A., Eltazi I.Z.M., Omer Z., Malik H., Mohamed M.O.E., Elhassan A.A., Mohamed E.O.E., Ahmed A.K.M.A., Ahmed E.A.A., Eltaraifee E., Hussein B.K., Abd Allah A.S.I., Salah L., Nimir M., Tag Elseed O.M., Elhassan T.E.A., Elbashier A., Alfadul E.S.A., Fadul M., Ali K.F., Taha S.O.M.A., Bushara E.E., Amin M., Koko M., Ibrahim M.E., Ahmed A.E., Elsayed L.E.O, & Stevanin G. (2023). Clinical phenotyping and genetic diagnosis of a large cohort of Sudanese families with hereditary spinocerebellar degenerations. European journal of human genetics : EJHG.

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