Plan fluor 20 0.50 na or 40 0 75 na objective lens

Images were taken at RT using the fluorochromes DAPI, Alexa Fluor 488 (green), and Alexa Fluor 555 (red). Fluorescence images were acquired with a microscope (Eclipse50i) equipped with a Plan Fluor 20×/0.50 NA or 40×/0.75 NA objective lens (Nikon), a camera (EXi Aqua; QImaging), and Image-Pro Express software (version 6.3; Media Cybernetics). Bright-field microscopy was performed using a microscope (Eclipse 50i; Nikon) equipped with a Plan Fluor 20×/0.50 NA or 40×/0.75 NA objective lens (Nikon), a camera (VisiCam 3.0; VWR International), and Visi-Cam Image Analyzer software (VWR International). No imaging medium was used. For the illustration purposes, images of the skin cryosections were adjusted using the levels and brightness/contrast tools in Photoshop (CS6; Adobe); the same adjustments were applied to every pixel in each RGB channel.

Cells were visualized with a 40×/0.55 (dry lens) objective using an inverted microscope (DIAPHOT 200; Nikon) at RT. Images were then captured with a digital camera (DS-Fi1; Nikon) using the autoexposure settings of NIS Elements BR 3.0 software (Nikon). For monitoring xenograft sections, bright field microscopy was performed at RT using a microscope (Eclipse 50i; Nikon) equipped with a Plan Fluor 20×/0.50 NA or 40×/0.75 NA objective lens (Nikon), a camera (VisiCam 3.0; VWR International), and VisiCam Image Analyzer software (VWR International). No imaging medium was used. After image acquisition, the images were color corrected using the match color command in Photoshop (CS 6.0; Adobe) to normalize for some exposure and lighting variations if the images for comparison were significantly different. This adjustment was applied to every pixel in each image.