Production of human IL-1β and IL-18 by vector-infected MDDCs or THP-1 cells was quantified using the LEGENDplex assay (Biolegend) following the manufacturer’s instruction. Briefly, cell-free supernatant was collected 48 hours post-infection and then incubated with Legendplex beads for 2 hours at room temperature on a plate shaker, followed by incubation with detection antibodies for 1 hour. After incubating with SA-PE for 30 min, beads were acquired on an LSR-II flow cytometer. Raw data were analyzed using the LEGENDplex™ software (Biolegend). Mouse IL-1β and IL-18 in supernatants of vector-infected mouse BMDCs were measured using the Mouse IL-1β ELISA MAX™ Deluxe (Biolegend) and Mouse IL-18 Platinum ELISA (eBioscience) according to the manufacturer’s instruction.
Mouse il 1β elisa max deluxe
Manufactured by BioLegend
The Mouse IL-1β ELISA MAX™ Deluxe is an enzyme-linked immunosorbent assay (ELISA) kit designed to quantitatively measure mouse interleukin-1 beta (IL-1β) levels in cell culture supernatants, serum, and plasma.
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2 protocols using mouse il 1β elisa max deluxe
1
Quantifying Cytokine Production in Immune Cells
2
Serum TNF-α and IL-1β Quantification
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TNF-α serum levels were determined by collecting blood samples in minicollect serum/plasma gel tubes (Greiner bio-one, Cat# 450472). After collection, samples were placed at room temperature for 30 min, centrifuged (10 min, 2000 RCF, 4 °C) and serum was collected. ELISAs were performed according to the manufacturer's instructions (Mouse TNF-α ELISA MAX™ Deluxe, Biolegend, Cat# 430906; Mouse IL-1β ELISA MAX™ Deluxe, Biolegend, Cat# 432606).
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