An aortic arch obtained from 6 week-old C57BL/6J WT or Ccn1-TG mice was cut into ~1.0 mm pieces. The aortic rings were placed in 48-well Matrigel or collagen-coated plates. Then, 100 μL of EBM-2 medium containing VEGF or CCN1 was added to each well. The formation of vascular sprouts was observed after 7 days. The ECs expressing with DLL4, CD34 and CD31 were determined as tip cells by immunofluorescent assay and counted under the confocal microscope (Leica TCS SP5 II Dichroic/CS, Germany).
Tcs sp5 2 dichroic cs
Manufactured by Leica
Sourced in Germany
The TCS SP5 II Dichroic/CS is a laser scanning confocal microscope system from Leica. It is designed to provide high-resolution imaging capabilities for a variety of scientific applications. The system features advanced optics, a flexible configuration, and high-performance detection capabilities.
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Lab products found in correlation
2 protocols using tcs sp5 2 dichroic cs
1
Aortic Angiogenesis Assay Utilizing Murine Tissue
2
Immunofluorescence Analysis of Aortic Cells
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Aorta, spheroids or cells after treatment with or without CCN1 were fixed with 4% paraformaldehyde (PFA) and incubated overnight with primary antibodies. The next day, they were washed several times and further incubated with secondary antibodies with specific fluorescent probes and visualised using a confocal microscope (Leica TCS SP5 II Dichroic/CS, Germany).
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