HUVECs (1 × 105) were plated in the top chamber of 6.5‐mm/8.0‐μm 0.2% gelatin‐coated Transwells (Corning) overnight. The endothelial monolayer was pretreated with either AB‐Tie1‐39 or IgG (10 μg/ml) for 24 h. Thereafter, LLC‐RFP cells (1 × 105) were seeded in the top chamber in DMEM containing 10% FCS with DMEM containing 10% FCS also in the bottom chamber. Transwells were washed 8 h later and fixed with Roti Histofix (4% PFA) for 10 min. Transmigrated LLC‐RFP cells were counted under a fluorescence microscope.
Gelatin coated transwells
Manufactured by Corning
Gelatin-coated Transwells are a specialized lab equipment designed for cell culture applications. They feature a porous membrane coated with gelatin, which facilitates cell attachment and growth. The Transwell system allows for the co-culture of different cell types or the study of cell migration and permeability.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using gelatin coated transwells
1
Endothelial Barrier Transmigration Assay
2
Permeability Assay for Cerebend Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Wild type (WT) and PcdhgC3 knockout (KO) cerebEND cells were grown on gelatin-coated transwells (pore size 0.4 µm, Corning) for 6 days. The permeability assay was performed as previously described (Curtaz et al., 2020 (link)) using 1 µM fluorescein (376 kDa, Sigma-Aldrich) for 1 h with aliquots taken from the basolateral compartment every 20 min. A parallel assay with cell-free transwells was used to calculate the endothelial permeability coefficient (Pe). The Pe of KO cells was normalized to WT cells.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!