Femoral and tibial bone marrow was isolated as described (Schulze, 2016 ). Bone-marrow cells were stained with FITC-conjugated rat anti-mouse CD41 or rat-IgG isotype control (both from BD Biosciences). Cells were washed in phosphate buffered saline (PBS) followed by fixation with 4% paraformaldehyde at room temperature for 1 h. After washing twice with PBS, cells were incubated with 5 μg/ml of DAPI and analyzed by flow cytometry (Trakala et al., 2015 (link)). HEL cells were stained with FITC-conjugated mouse anti-human CD41 (BD Biosciences), followed by flow cytometry analysis as above.
Fitc conjugated rat anti mouse cd41
Manufactured by BD
FITC-conjugated rat anti-mouse CD41 is a fluorescently labeled antibody that binds to the mouse CD41 antigen. CD41 is a cell surface glycoprotein that is expressed on platelets and megakaryocytes. This product can be used for the identification and enumeration of CD41-positive cells in flow cytometry applications.
Automatically generated - may contain errors
2 protocols using fitc conjugated rat anti mouse cd41
1
Immunophenotyping of Hematopoietic Cells
2
Fetal Liver Cryosectioning and Immunostaining
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Fetal liver on embryonic day 13.5 was isolated, fixed with 4% paraformaldehyde and subjected to perform cryosectioning. The sections were co-stained with FITC-conjugated rat anti-mouse CD41 (BD Biosciences) and polyclonal antibodies against Y14 (Bethyl) or γH2AX (Novus) overnight at 4°C, and then were then incubated with appropriate secondary antibodies and stained with Hoechst 33258 (Sigma).
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