a) Pure samplesIpriflavone (99.64%), ascorbic acid (100.17%), pyridoxine hydrochloride (99.8%), vitamin D3 oil 40,000,000 IU/gm and lysine hydrochloride (99.66%) were kindly obtained from Eldebeiky Pharma (DBK), Egypt.
b) Market samplesUltracalce® capsules labeled to contain ipriflavone 25 mg, ascorbic acid 30 mg, pyridoxine hydrochloride 1 mg, vitamin D3 0.005 mg and lysine hydrochloride 25 mg per capsule manufactured by AL-Debeiky Pharma Co. were purchased from the local market.
c) Chemicals and reagentsHPLC grade methanol (Scharlau, Spain), ammonium acetate (Qualikems, India), glacial acetic acid (Merck, Germany), sodium dihydrogen phosphate, sodium chloride and phosphoric acid (Merck, Germany) and Merck Milli Q Ultrapure Water were used in chromatographic analyses. Twenty-five millimole ammonium acetate buffer was prepared by dissolving 1.93 g of ammonium acetate in one litre of water (pH was adjusted to 4.2 with glacial acetic acid). The mobile phase for lysine hydrochloride elution was prepared by dissolving 1.56 gm sodium dihydrogen phosphate and 11.9 gm sodium chloride in one litre water (pH was adjusted to 6 with orthophosphoric acid).
Galal S.A., Elzanfaly E.S., Hussien E.M., Amer E.A, & Zaazaa H.E. (2024). Development of chromatographic methods to determine multivitamins formulation depending on their solubility and polarity: comparative study using three greenness assessment tools. BMC Chemistry, 18(1), 20.
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