All reagents were purchased at Sigma (Munich, Germany) unless otherwise indicated. 4-Styrenesulfonic acid sodium salt hydrate (NaSS) was from Sigma-Aldrich (cat. no. 434574). The anti-SOX9 (C-20) antibody was from Santa Cruz Biotechnology (Heidelberg, Germany), the anti-type-II collagen (AF-5710) and anti-type-I collagen (AF-5610) antibodies from Acris (Hiddenhausen, Germany), and the anti-type-X collagen (COL-10) antibody from Sigma. The biotinylated secondary antibodies and ABC reagent were from Vector Laboratories (Alexis Deutschland GmbH, Grünberg, Germany). The AAVanced Concentration Reagent was from System Bioscience (Heidelberg, Germany) and the Cell Proliferation Reagent WST-1 from Roche Applied Science (Mannheim, Germany).
4 styrenesulfonic acid sodium salt hydrate nass
Manufactured by Merck Group
4-Styrenesulfonic acid sodium salt hydrate (NaSS) is a chemical compound that serves as a laboratory reagent. It is a white to off-white crystalline solid that is soluble in water. NaSS is commonly used as a monomer in the synthesis of various polymers and copolymers.
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Lab products found in correlation
Biotinylated secondary antibody, by Vector Laboratories (1 mentions)
Aavanced concentration reagent, by System Biosciences (1 mentions)
Abc reagent, by Vector Laboratories (1 mentions)
Anti sox9 c 20 antibody, by Santa Cruz Biotechnology (1 mentions)
Anti type x collagen col 10 antibody, by Merck Group (1 mentions)
Cell proliferation reagent wst 1, by Roche (1 mentions)
Hydrochloric acid (hcl), by Thermo Fisher Scientific (1 mentions)
Ammonium hydroxide nh4oh, by Thermo Fisher Scientific (1 mentions)
2 2 0 azobis 2 methylpropionitrile aibn, by Merck Group (1 mentions)
Divinylbenzene dvb, by Merck Group (1 mentions)
3 trimethoxysilyl propyl methacrylate tpm, by Merck Group (1 mentions)
Ethanol etoh, by Avantor (1 mentions)
4 tert butylcatechol, by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
2 protocols using 4 styrenesulfonic acid sodium salt hydrate nass
1
Chondrogenic Differentiation Assay
2
Fluorescent Polymer Synthesis Protocol
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Materials 4-Styrenesulfonic acid sodium salt hydrate (NaSS, Sigma-Aldrich), 2,2 0 -azobis(2-methylpropionitrile) (AIBN, 98%, Sigma-Aldrich), methanol (MeOH, absolute HPLC, Biosolve Chimie), divinylbenzene (DVB, technical grade 80%, Sigma-Aldrich), methacryloxyethyl thiocarbamoyl rhodamine B (PolyFluor s 570, Polysciences), ammonium hydroxide (NH 4 OH, 28-30 wt% in water, Acros Organics), 3-(trimethoxysilyl)propyl methacrylate (TPM, Z97%, Sigma-Aldrich), dimethyl sulfoxide (DMSO, Sigma-Aldrich), gellan gum (gellan, powder, Alfa Aesar), ethanol (EtOH, absolute, VWR Chemicals), Norland optical adhesive 81 (UV glue, Norland), pyrromethene BODIPY TM 493/503 (BODIPY, Invitrogen TM ), potassium chloride (KCl, Z99%, Acros Organics), n-decane (Z99%, Carl Roth GmbH) and hydrochloric acid (HCl, 37% in water, Acros Organics) were used as received. Styrene (Z99%, containing 4-tert-butylcatechol as a stabilizer, Sigma-Aldrich) was purified through a column packed with silica gel (high purity grade: Davisil Grade 633, Sigma-Aldrich) and aluminum oxide powder (Z98%, Honeywell Fluka). The water used in all experiments was purified by filtration through Millipore filters (18.2 MO cm at 25 1C).
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