The Smac-mimetic Compound A, the caspase inhibitor IDN-6556 (Idun Pharmaceuticals) and the RIPK1 inhibitor Necrostatin were synthesized by TetraLogic Pharmaceuticals. The RIPK3 inhibitor GSK’872 was from Calbiochem. The TAK1 inhibitor (5Z)-7-Oxozeaenol, the IKK inhibitor IKK-16 and the MK2 inhibitor PF-3644022 were from Tocris Bioscience. Cycloheximide was from Sigma. Recombinant Fc-TNF was produced in house. Ultrapure LPS-EB and poly(I:C) were purchased from Invivogen.
Tak1 inhibitor 5z 7 oxozeaenol
Manufactured by Bio-Techne
Sourced in United States
(5Z)-7-Oxozeaenol is a TAK1 (Transforming Growth Factor-beta-Activated Kinase 1) inhibitor. TAK1 is a serine/threonine protein kinase that plays a role in various cellular processes. (5Z)-7-Oxozeaenol functions as a selective and potent inhibitor of TAK1 activity.
Automatically generated - may contain errors
Lab products found in correlation
Lps eb ultrapure, by InvivoGen (3 mentions)
Cycloheximide (chx), by Merck Group (3 mentions)
Poly 1 c, by InvivoGen (2 mentions)
Pf 3644022, by Bio-Techne (2 mentions)
Ikk16, by Bio-Techne (2 mentions)
Dual glo luciferase assay system, by Promega (1 mentions)
Renilla luciferase plasmid, by Promega (1 mentions)
Nigericin, by Merck Group (1 mentions)
Mcc950, by Merck Group (1 mentions)
Vx 765, by MedChemExpress (1 mentions)
4 protocols using tak1 inhibitor 5z 7 oxozeaenol
1
Modulation of Cell Death Pathways
2
Modulation of Cell Death Pathways
3
Regulation of AP-1 and IκB-α in RCC
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For luciferase assay, RCC cell lines were transfected with the AP-1 or IκB-α reporter vector and a Renilla luciferase plasmid (Promega, Madison, WI, United States) as a control for transfection efficiency. To study whether AP-1 activation is regulated by TAK1/JNK pathway in RCC cells, the transfected cells were pretreated with TAK1-inhibitor 5Z-7-oxozeaenol (0, 50, 100, 200 nM) (Tocris Bioscience, Ellisville, MO, United States) or JNK-inhibitor II SP600125 (0, 2.5, 5, 10 µM) (Calbiochem, San Diego, CA, United States) for 1 h. Furthermore, to study the possible role of AP-1 and IκB-α in anticancer activities of NVP-BEZ235, PP242, and Rapamycin, the transfected cells were treated with these compounds (10, 100, 1,000 nM) for 24 h, or the equivalent amounts of DMSO as control. Luciferase activity was measured using a Dual-Glo® Luciferase Assay System (Promega) according to the manufacturer’s protocol.
4
Investigating Cell Death Pathways
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The Smac-mimetic compound A and the caspase inhibitor IDN-6556 (Idun Pharmaceuticals) were synthesised by TetraLogic Pharmaceuticals. The RIPK3 inhibitor GSK’872 was from Calbiochem. The TAK1 inhibitor (5Z)-7-oxozeaenol was from Tocris Bioscience. Cycloheximide and nigericin were from Sigma. The NLRP3 inhibitor MCC950 was purchased from Merck. Caspase-1-specific inhibitor Vx765 was from MedChemExpress. Ultrapure LPS-EB, poly(I:C), P3Cys, and CpG were purchased from Invivogen.
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