Unroofed cells were prepared as for correlative microscopy. Intact cells were rinsed with imaging buffer and fixed with 2% paraformaldehyde. Immunolabeling clathrin heavy chain with Atto 488 and epsin 1 with AF647 is described above. Fixed and immunolabeled cells were placed in blinking buffer and imaged with a Nikon N-STORM super-resolution microscope system (N-1245) equipped with a CFI SR Apochromat TIRF 100x oil objective, a 80mW 488nm laser, a 125 mW 647 nm laser, and an Andor Ixon Ultra DU-897 camera. Two-color images were acquired in TIRF at full laser power with 20 ms frames. Final super-resolution images were localizations from 20–30,000 frames (each color).
Ultra du 897 camera
Manufactured by Oxford Instruments
The Ultra DU-897 camera is a high-performance scientific camera designed for demanding imaging applications. It features a large-format CCD sensor, low-noise electronics, and advanced cooling technology to deliver exceptional image quality and sensitivity. The camera's core function is to capture and record images with high spatial and temporal resolution, making it suitable for a wide range of scientific and industrial applications.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using ultra du 897 camera
1
Super-resolution Imaging of Clathrin and Epsin
2
Super-resolution Imaging of Clathrin and Epsin
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