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Cytotox one c homogeneous membrane integrity assay kit

Manufactured by Promega
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Sourced in United States

The CytoTox-ONE™ Homogeneous Membrane Integrity Assay Kit is a quantitative, fluorometric-based assay that measures the release of lactate dehydrogenase (LDH) from cells with damaged membranes. The assay provides a simple, reproducible method for determining the number of non-viable cells in a cell population.

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CytoTox-ONE™ Homogeneous Membrane Integrity Assay (135 citations) CytoTox-ONE™ Homogeneous Membrane Integrity Assay kit (36 citations) CytoTox-ONE™ kit (24 citations) CytoTox-ONE (24 citations) CytoTox-ONE assay (19 citations) CytoTox® Kit (16 citations) CytoTox-ONETM Homogeneous Membrane Integrity Assay (10 citations) CytoTox-ONETM Homogeneous Membrane Integrity Assay Kit (5 citations) CytoTox-ONE assay kit (3 citations) CytoTox-ONE Homogeneous Membrane Integrity kit (2 citations)

2 protocols using cytotox one c homogeneous membrane integrity assay kit

1

Cytotoxicity Assay Using LDH

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Cytotoxicity was detected using a CytoTox-ONE™ C homogeneous membrane integrity assay kit (Promega, USA), a fluorometric method used to measure the amount of lactate dehydrogenase (LDH) released into the medium from inactive cells. All experiments were performed according to the manufacturer’s instructions. The percentage of LDH released into the medium was used to evaluate cytotoxicity.
Feng Y.H., Li L.F., Zhang Q., Zhang J.H., Huang Y., Lv Y.L., Jia J.Z., Zhang D., Hu J.Y, & Huang Y.S. (2021). Microtubule associated protein 4 (MAP4) phosphorylation reduces cardiac microvascular density through NLRP3-related pyroptosis. Cell Death Discovery, 7, 213.
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2

Cytotoxicity Detection via LDH Assay

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Cytotoxicity was detected with a CytoTox-ONE C homogeneous membrane integrity assay kit (Promega, Madison, WI, United States), which is a fluorometric method used to measure the amount of LDH released into the medium from inactive cells. All experiments were performed according to the manufacturer’s instructions and repeated three times. The percentage of LDH released into the medium were used to reflect cytotoxicity.
Huang Y., Feng Y., Cui L., Yang L., Zhang Q., Zhang J., Jiang X., Zhang X., Lv Y., Jia J.Z., Zhang D.X, & Huang Y.S. (2021). Autophagy-Related LC3 Accumulation Interacted Directly With LIR Containing RIPK1 and RIPK3, Stimulating Necroptosis in Hypoxic Cardiomyocytes. Frontiers in Cell and Developmental Biology, 9, 679637.
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