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Porcine intestinal mucosa heparin

Manufactured by Merck Group
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Sourced in Germany

Porcine intestinal mucosa heparin is a raw material derived from the intestinal mucosa of pigs. It is a complex mixture of glycosaminoglycans, primarily containing heparin.

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Lab products found in correlation

Eclia assay, by Roche (1 mentions) Calcium ionophore a23187, by Merck Group (1 mentions) Goat anti rat alexa 647 antibody, by Thermo Fisher Scientific (1 mentions) Recombinant human cytokines and chemokines, by Immunotools (1 mentions) Phorbol myristate acetate (pma), by Merck Group (1 mentions)

Spelling variants of this product

Heparin (2033 citations) Heparin sodium salt from porcine intestinal mucosa (36 citations) Heparin from porcine intestinal mucosa (13 citations) Porcine heparin (12 citations) Porcine intestinal mucosa (4 citations) Heparin sodium salt from porcine intestinal mucosa Grade I-A (3 citations) Porcine intestinal heparin (2 citations) Heparin sodium salt from the porcine intestinal mucosa (2 citations)

3 protocols using porcine intestinal mucosa heparin

1

Recombinant Histones and Antibody Preparation

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Recombinant human β-tryptase was prepared as described [6 (link)]. Recombinant human histones (H2A, H2B, H3, H4) were purchased from New England Biolabs (Ipswich, MA, USA). Porcine intestinal mucosa heparin was from Sigma-Aldrich (Steinheim, Germany). Rabbit anti-histone H2A, H2B, H3 and H4 polyclonal antibodies were from Abcam (Cambridge, UK).
Alanazi S., Grujic M., Lampinen M., Rollman O., Sommerhoff C.P., Pejler G, & Melo F.R. (2020). Mast Cell β-Tryptase Is Enzymatically Stabilized by DNA. International Journal of Molecular Sciences, 21(14), 5065.
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2

Optimized THBS2 ELISA for Plasma Biomarker

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The THBS2 ELISA kit from Bio-Techne (R&D Systems DTSP20) was used as per the recommendations of the manufacturer except that to minimize lot-to-lot variation in the kit’s diluent buffer RD1–75, we developed and used our own diluent buffer consisting of 0.2 mg/ml porcine intestinal mucosa heparin (Sigma #H3149) in protein-free phosphate-buffered saline with Tween 20 (Thermo Fisher #37573). Manual THBS2 ELISAs were performed on plasma samples with the new diluent buffer as otherwise described previously (5 ) and automated ELISAs were performed on a Dynex DS2 device in a pre-clinical biomarker laboratory, working with the Dynex to ensure reproducible and accurate performance (see Figure 1). CA19–9 levels were detected with a clinical ECLIA assay (Roche) at the Mayo Clinic.
Udgata S., Takenaka N., Bamlet W.R., Oberg A.L., Yee S.S., Carpenter E.L., Herman D., Kim J., Petersen G.M, & Zaret K.S. (2020). THBS2/CA19–9 detecting pancreatic ductal adenocarcinoma at diagnosis underperforms in pre-diagnostic detection: Implications for biomarker advancement. Cancer prevention research (Philadelphia, Pa.), 14(2), 223-232.
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3

Neutrophil Activation Analysis Protocol

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Recombinant human (rh) cytokines and chemokines were purchased from ImmunoTools (Friesoythe, Germany). rh β-tryptase was prepared as described [35 (link)]. Porcine intestinal mucosa heparin, phorbol myristate acetate (PMA), calcium ionophore A23187, and lipopolysaccharide from Escherichia coli O111:B4 (lipopolysaccharide [LPS]) were from Sigma-Aldrich (Steinheim, Germany). N-formyl-Met-Leu-Phe (fMLP) was from Tocris (Abingdon, UK). NucBlueTM live ReadyProbesTM reagent (Hoechst-33342), NucGreenTM dead 488 ReadyProbesTM reagent (Sytox-green), ProLongTM diamond antifade mountant, goat anti-rat Alexa-647 antibody, goat anti-rabbit Alexa-555 antibody and BS3 (bis[sulfosuccinimidyl]suberate) were from Thermo Fisher Scientific (Waltham, MA, USA). Rat anti-mouse Ly-6G and Ly-6C antibody were from BD-Pharmingen (San Jose, CA, USA), rabbit anti-human histone histone 2A (H2A), H2B, H3, and H4 antibodies were from Abcam (Cambridge, UK) and rabbit anti-human histone H3 citrulline Arg2, −8 and −17 antibody was from Novus (Centennial, CO, USA).
Pejler G., Alanazi S., Grujic M., Adler J., Olsson A.K., Sommerhoff C.P, & Rabelo Melo F. (2021). Mast Cell Tryptase Potentiates Neutrophil Extracellular Trap Formation. Journal of Innate Immunity, 14(5), 433-446.
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