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Epiquik m6a m rna methylation quantification kit

Manufactured by Epigentek
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The EpiQuik m6A/m RNA Methylation Quantification Kit is a lab equipment product that provides a fast and convenient way to quantify the levels of N6-methyladenosine (m6A) and other RNA methylation markers in various RNA samples.

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EpiQuik m6A RNA Methylation Quantification Kit (150 citations) M6A RNA methylation quantification kit (38 citations) EpiQuik m6A methylation quantification kit (6 citations) EpiQuik m6A RNA Methylation kit (4 citations) EpiQuikTM (2 citations)

4 protocols using epiquik m6a m rna methylation quantification kit

1

Quantifying Global m6A RNA Methylation

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Global m6A/m in total RNA was quantified by the EpiQuik m6A/m RNA Methylation Quantification Kit (Epigentek Group, Farmingdale, NY) following manufacturers’ specifications and using 100-300 ng input (in duplicates or triplicates). Comparing total RNA global m6A/m measurements with LC-MS/MS data from the same conditions, we observed high correlation of stress-changes, suggesting that the total RNA colorimetric assay represents an appropriate tool to detect global m6A/m regulation patterns. Brain global methylation in PFC and AMY is not regulated by circadian rhythm (data not shown).
Engel M., Eggert C., Kaplick P.M., Eder M., Röh S., Tietze L., Namendorf C., Arloth J., Weber P., Rex-Haffner M., Geula S., Jakovcevski M., Hanna J.H., Leshkowitz D., Uhr M., Wotjak C.T., Schmidt M.V., Deussing J.M., Binder E.B, & Chen A. (2018). The Role of m6A/m-RNA Methylation in Stress Response Regulation. Neuron, 99(2), 389-403.e9.
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2

Quantifying Total m6A RNA Methylation

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To measure total m6A methylation level in total RNA, the EpiQuik m6A/m RNA Methylation Quantification Kit (Epigentek Group, Farmingdale, NY) was employed followed by the manufacturer's instruction. Briefly, 500 ng of total RNA was involved in each reaction. Labelled total RNA was analysed using LC–MS/MS.
Li L., Chen J., Wang A, & Yi K. (2023). ALKBH5 regulates ovarian cancer growth via demethylating long noncoding RNA PVT1 in ovarian cancer. Journal of Cellular and Molecular Medicine, 28(2), e18066.
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3

Quantifying m6A RNA Methylation

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The global m6A/m in total RNA was detected with the EpiQuik m6A/m RNA Methylation Quantification Kit (Epigentek Group, Farmingdale, NY, USA) following manufacturers’ specifications with 100–300 ng input RNA (in triplicate).
Mu X., Zhao Q., Chen W., Zhao Y., Yan Q., Peng R., Zhu J., Yang C., Lan K., Gu X, & Wang Y. (2021). IL-37 Confers Anti-Tumor Activity by Regulation of m6A Methylation. Frontiers in Oncology, 10, 526866.
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4

Quantification of m6A/m in RNA

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Global m6A/m in total RNA was quantified by the EpiQuik m6A/m RNA Methylation Quantification Kit (Epigentek Group) following the manufacturers’ specifications and using 100 ng as input.
Chang C., Ma G., Cheung E, & Hutchins A.P. (2022). A programmable system to methylate and demethylate N6-methyladenosine (m6A) on specific RNA transcripts in mammalian cells. The Journal of Biological Chemistry, 298(11), 102525.
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