Thiobarbituric acid reactive substances assay

Oxidative stress was evaluated by assessing the contents of malondialdehyde (MDA), superoxide-dismutase (SOD) and glutathione peroxidase (GSH-Px). MDA content was assessed using thiobarbituric acid reactive substances assay (Jiancheng Bioengineering, Nanjing, China) by measuring the absorbance value at wavelength of 532 nm. SOD activity was assessed using the xanthine oxidase method to measure the absorbance value at 550 nm with a SOD kit (Jiancheng Bioengineering, Nanjing, China). GSH-Px activity was estimated by the analysis of glutathione in the enzymatic reaction. Reduction of GSH is catalyzed by GSH-Px in the presence of hydrogen peroxide. One unit of enzyme activity represents a decrease in GSH concentration of 1 μm/ml of serum.

To evaluate the oxidative stress, the serum contents of SOD, glutathione peroxidase (GSH-Px) and MDA. SOD activity was assessed with the xanthine oxidase method and the absorbance value was measured at 550 nm with a SOD kit (Jiancheng Bioengineering, Nanjing, China). MDA content was assessed with thiobarbituric acid reactive substances assay (Jiancheng Bioengineering, Nanjing, China) and the absorbance value was measured at a wavelength of 532 nm. GSH-Px activity was estimated by the analysis of glutathione in the enzymatic reaction. GSH-Px catalyzed the reduction of GSH in the presence of hydrogen peroxide. One unit of enzyme activity represents a decrease in GSH concentration of 1 μm/ml of serum (Yang et al., 2015 (link)).