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Instantblue coomassie

Manufactured by Merck Group
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InstantBlue Coomassie is a ready-to-use protein stain solution designed for fast and sensitive detection of proteins in gels. It provides a simple, one-step staining procedure with no destaining required.

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Lab products found in correlation

Nanodrop nd 1000, by Thermo Fisher Scientific (1 mentions) Nanodrop nd 1000 spectrophotometer, by Thermo Fisher Scientific (1 mentions)

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InstantBlue (43 citations) InstantBlue Coomassie stain (4 citations)

2 protocols using instantblue coomassie

1

SDS-PAGE Protein Separation and Quantification

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Proteins were separated by using 4%–12% TruPAGE precast gels SDS-PAGE gels (Merck KGaA) loaded with 5 μg of protein at a 3:1 mix of sample to loading buffer and stained using InstantBlue Coomassie according to manufacturer’s instructions (Merck KGaA). Protein concentration was determined using a Spectrophotometer (NanoDrop ND-1000, NanoDropTechnologies) at absorbance 280 nm.
Hofherr A., Seger C., Fitzpatrick F., Busch T., Michel E., Luan J., Osterried L., Linden F., Kramer-Zucker A., Wakimoto B., Schütze C., Wiedemann N., Artati A., Adamski J., Walz G., Kunji E.R., Montell C., Watnick T, & Köttgen M. (2018). The mitochondrial transporter SLC25A25 links ciliary TRPP2 signaling and cellular metabolism. PLoS Biology, 16(8), e2005651.
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2

SDS-PAGE Protein Separation and Quantification

Check if the same lab product or an alternative is used in the 5 most similar protocols
Proteins were separated using precast 4%–12% TruPAGE™ SDS–PAGE gels (Merck KGaA), loaded with 5 µg of protein at 3:1 mix of sample to loading buffer and stained using InstantBlue™ Coomassie (Merck KGaA). Protein concentration was determined by measuring the absorbance at 280 nm using a NanoDrop ND‐1000 Spectrophotometer (NanoDropTechnologies).
Jabalameli M.R., Fitzpatrick F.M., Colombo R., Howles S.A., Leggatt G., Walker V., Wiberg A., Kunji E.R, & Ennis S. (2021). Exome sequencing identifies a disease variant of the mitochondrial ATP‐Mg/Pi carrier SLC25A25 in two families with kidney stones. Molecular Genetics & Genomic Medicine, 9(12), e1749.
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