Culture medium and

Culture medium and supplements were purchased from Thermo Fisher Scientific (Waltham, MA, USA). Oleate, palmitate, palmitOleate, linOleate, stearate, cycloheximide, actinomycin D, HepG2, and HEK293T cells were purchased from Sigma-Aldrich (St. Louis, MO, USA). Polyclonal primary antibodies against SCD1 and Actin and secondary antibodies were obtained from Cell Signaling (Danvers, MA, USA). Antibody against Glu-Glu tag was purchased from Bethyl Laboratories (Montgomery, TX, USA). Methanol (gradient grade), isopropanol (gradient grade), n-hexane, and boron trifluoride-methanol solution were purchased from Merck (Rahway, NJ, USA). All other chemicals used in this study were of analytical grade. All experiments and measurements were carried out by using Millipore ultrapure water.

Culture medium and supplements were purchased from Thermo Fisher Scientific (Waltham, MA, USA). Oleate, palmitate, stearate, linOleate, elaidate, vaccenate, bovine serum albumin, HEK293T and HepG2 cells were purchased from Sigma-Aldrich (St. Louis, MO, USA). All chemicals used in the study were of analytical grade. All experiments and measurements were performed using Millipore ultrapure water.