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Shrna hoxa as2 2

Manufactured by GenePharma
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Sourced in China

ShRNA-HOXA-AS2-2 is a laboratory tool designed for gene knockdown experiments. It is a short hairpin RNA (shRNA) targeting the HOXA-AS2 gene. The product is intended for use in cell-based assays and research applications to modulate the expression of the HOXA-AS2 gene.

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Lab products found in correlation

Shrna hoxa as2 1, by GenePharma (2 mentions) Shrna nc, by GenePharma (2 mentions) Lipofectamine 3000 reagent, by Thermo Fisher Scientific (2 mentions) Shrna nc, by Thermo Fisher Scientific (1 mentions) Pcdna nc, by Sangon (1 mentions) Pcdna nc, by Thermo Fisher Scientific (1 mentions)

2 protocols using shrna hoxa as2 2

1

HOXA-AS2 Regulation in Tca-8113 Cells

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The short-hairpin RNA (shRNA) against HOXA-AS2 (shRNA-HOXA-AS2-1 and shRNA-HOXA-AS2-2) and corresponding empty vector (shRNA-NC) were purchased from GenePharma. The pcDNA-EZH2 and the empty vector pcDNA-NC were constructed from Sangon Biotech. Tca-8113 cells (1 × 106 cells/well) were inoculated into 12-well plates and then transfected with shRNA-NC, shRNA-HOXA-AS2, pcDNA-NC, and pcDNA-EZH2 using Lipofectamine® 3000 reagent (Thermo Fisher Scientific) following the manufacturer’s instruction. After 48 h, RT-qPCR was used to assess the transfection efficiencies. The transfected cells were collected for the next experiments.
Zhao Z., Xing Y., Yang F., Zhao Z., Shen Y., Song J, & Jing S. (2021). LncRNA HOXA-AS2 Promotes Oral Squamous Cell Proliferation, Migration, and Invasion via Upregulating EZH2 as an Oncogene. Technology in Cancer Research & Treatment, 20, 15330338211039109.
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2

Knockdown of HOXA-AS2 in TCA-8113 Cells

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Short-hairpin RNA (shRNA) against HOXA-AS2 (shRNA HOXA-AS2-1, shRNA HOXA-AS2-2) and corresponding empty vector (shRNA NC) were purchased from GenePharma (Shanghai, China). Liposome 3000 transfection reagent (Thermo Fisher Scienti c) were used to transfect into TCA-8113 cells following the product instruction. After 48 hours, qRT-PCR was used to detect.
Zhao Z., Xing Y., Yang F., Zhao Z., Shen Y., Song J., & Jing S. (2020). Knowdown of lncRNA HOXA-AS2 inhibits proliferation, invasion and migration of oral squamous cell carcinoma.
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