CD44 expression on prostate cells was evaluated using flow cytometry. Experiments were performed with FITC-conjugated mouse anti-human CD44 antibodies (BD Biosciences) using a FACS Canto II instrument (BD Biosciences). Data were analyzed with Cell Quest software (BD Biosciences).
Fitc conjugated mouse anti human cd44 antibody
Manufactured by BD
Sourced in United States
FITC-conjugated mouse anti-human CD44 antibodies are a laboratory reagent used for the detection and identification of the CD44 cell surface antigen in human cells. The antibody is conjugated with the fluorescent dye FITC, allowing for the visualization and analysis of CD44-expressing cells.
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4 protocols using fitc conjugated mouse anti human cd44 antibody
1
Quantifying CD44 Expression in Prostate Cells
2
Flow Cytometric Analysis of CD44 Expression
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CD44 expression on prostate cells was evaluated using ow cytometry. Experiments were performed with FITC-conjugated mouse anti-human CD44 antibodies (BD Biosciences, San Diego, CA, USA) using a FACS Canto II instrument (BD Biosciences, San Diego, CA, USA). Data were analyzed with Cell Quest software (BD Biosciences).
3
CD44 Expression Analysis by Flow Cytometry
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Cells were harvested and washed 3 times with ice‐cold PBS containing 0.25% FBS. Cells were incubated with FITC‐conjugated mouse‐anti‐human CD44 antibody (BD, 555478) or FITC‐conjugated mouse IgG2b antibody (BD, 555742) for 30 min at 4°C in the dark. Cells were then washed thrice with PBS, run through a Beckman Coulter CytoFLEX flow cytometer at 488 nm, and analyzed using CytExpert V1.2 software.
4
CD44 Expression in TS/A-pc and HeLa Cells
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To measure expression of the HA-binding receptor CD44 in TS/A-pc and HeLa cells, TS/A-pc and HeLa cells were seeded into a 6well plate at a density of 5 × 10 5 cells per well and incubated for 24 h at 37 °C in a humidified atmosphere of 5% CO2. Cells were trypsinated, counted and washed before incubation for 1 h in 300 µL of PBS containing 10 µL of fluorescein isothiocyanate (FITC)conjugated mouse anti-human CD44 antibody (#555478 from BD Pharmingen) at 4 °C. FITC mouse IgG2b κ antibody was used as isotype control (# 555742 from BD Pharmingen). After three washing with PBS, the cells were observed using fluorescence-activated cell sorting (FACS) Accuri 6 (Becton, Dickinson and Company, Pont de Claix, France). Cells were gated to exclude cell debris and 10,000 events were recorded in the gate for fluorescence intensity measurement.
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