Cells were laser-captured onto Zeiss PALM AdhesiveCap™ (Carl Zeiss GmbH, Germany) and RNA was extracted using the RNEasy® micro kit (Qiagen, Crawley, UK). The AdhesiveCap tubes were closed, inverted, and incubated for 30 minutes at room temperature. The lysates were then centrifuged at 12,000 rpm for 5 minutes. Samples were stored at −80°C for batch RNA extraction and purification. Total RNA was prepared using RNEasy® micro spin columns (Qiagen, Crawley, UK), with on-column DNase I digestion to remove genomic DNA contamination. RNA concentrations and qualities were assessed using the Agilent RNA 6000 Pico LabChip® on the Agilent Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA, USA). Samples with RNA integrity number (RIN) < 5 were considered of insufficient quality and excluded from downstream RNA amplification, biotinylation, and gene expression microarray analysis.
Agilent rna 6000 pico labchip
Manufactured by Agilent Technologies
Sourced in United States
The Agilent RNA 6000 Pico LabChip is a microfluidic-based instrument used for the analysis of small amounts of RNA samples. It provides automated electrophoretic separation and fluorescence detection of RNA fragments, enabling the assessment of RNA quality and quantity.
Automatically generated - may contain errors
2 protocols using agilent rna 6000 pico labchip
1
Laser Capture Microdissection and RNA Extraction
2
RNA Extraction from Laser-Captured Cells
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Cells were laser captured onto Zeiss PALM AdhesiveCap (Carl Zeiss GmbH, Germany), and RNA was extracted using the RNEasy micro kit (Qiagen, Crawley, United Kingdom). The AdhesiveCap tubes were closed, inverted, and incubated for 30 minutes at room temperature. The lysates were then centrifuged at 12 000 rpm for 5 minutes. Samples were stored at −80°C for batch RNA extraction and purification. Total RNA was prepared using RNEasy micro spin columns (Qiagen, Crawley, United Kingdom), with on-column DNase I digestion to remove genomic DNA contamination. RNA concentrations and qualities were assessed using the Agilent RNA 6000 Pico LabChip on the Agilent Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA). Samples with RNA integrity number (RIN) <5 were considered of insufficient quality and excluded from downstream RNA amplification, biotinylation, and gene expression microarray analysis.
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