Meoh hplc grade

All solvents used during extraction procedures were analytical grade except for methanol (MeOH). Chromatographic separation was carried out using MeOH HPLC grade purchased from Roth (Carl Roth GmbH, Germany) or J. T. Baker (Xalostoc, Mexico). IAA (purity > 99%), ABA (purity > 99%) and SA (>98%) were purchased from Sigma-Aldrich. 12-oxo phytodienoic acid were purchased from Cayman (Biomol GmbH, Hamburg, Germany). JA was synthesized by saponification of commercially available methyl-JA. Jasmonic acid isoleucine conjugate (JA-Ile) was synthetized according to Kramell et al. (1988 (link)). Deuterated standards: [²H5] indole-3-acetic acid (d5-IAA), [²H4] salicylic acid (d4-SA) and [²H6] (+)-cis, trans-abscisic acid (d6-ABA) were purchased from OlChemIm Ltd (Olomouc, Czech Republic) and jasmonic-d5 acid 2,4,4-d3 acetyl-2,2-d2 (d5-JA) was purchased from CDN isotopes (Quebec, QC, Canada).

HPLC grade water and LC-MS grade methanal were purchased from Acros Organics (New Jersey). Lysophosphatidylcholine 17:1 (LPC 17:1) was purchased from Avanti Polar Lipids (Alabaster, AL). The extraction solvent used in this study was composed of 1 μM LPC 17:1 in 9 : 1 MeOH : H₂O (v/v). Fused silica capillaries (50 μm ID, 150 μm OD) were purchased from Polymicro Technologies (Phoenix, AZ). The MinuteWeld epoxy glue was purchased from J-B Weld company (Sulphur Springs, TX). A 2000 grit silicon carbide sandpaper (STARCKE, Germany) was used to polish the SLE-MFP sampling port.
4′′ fused-silica substrates with a thickness of 1 mm were obtained from Siegert Wafer GmbH (Aachen, Germany). Potassium hydroxide solution (45%, extra pure) and MeOH (HPLC grade) were purchased from Carl Roth GmbH & Co. KG (Karlsruhe, Germany). Dioctyl sulfosuccinate sodium salt (≥99%) was purchased from Sigma-Aldrich GmbH (Taufkirchen, Germany).