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The EHPGE2 is a high-performance germanium detector designed for gamma-ray spectroscopy applications. It provides accurate and reliable measurements of gamma-ray energies and intensities.

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Lab products found in correlation

2 protocols using ehpge2

1

Quantification of Secreted Inflammatory Factors

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The quantification of the secreted levels of IL-1α (BMS243-2 Thermo Fisher Scientific, Waltham, MA, USA), PGE2 (EHPGE2, Thermo Fisher Scientific), IL-6 (BMS213HS, Thermo Fisher Scientific), MMP-1 (RAB0361, Sigma Aldrich), and TIMP-1(RAB0466, Sigma Aldrich) was performed using commercially available ELISA kits according to the manufacturer’s instructions.
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2

Quantifying Cytokine Secretion in moMDSCs

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For the detection of IL-6, IL-10, and PGE2 production, harvested moMDSCs were replated in 96 U bottom plates at a concentration of 0.5 million cells per ml at a final volume of 200 µL of X-VIVO medium supplemented with 2% HS and 100 ng/ml LPS. After 24 hours of culture, the moMDSCs supernatants were harvested and analyzed by ELISA for the presence of IL-6 (88-7106-77, Invitrogen), IL-10 (88-7066-77 Invitrogen), and PGE2 (EHPGE2 ThermoFisher) according to manufacturer’s instructions.
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