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Sequencing grade modified trypsinwas

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Sequencing grade modified trypsin is a proteolytic enzyme used for the digestion of proteins. It is designed for use in DNA sequencing applications, providing consistent and reliable protein cleavage to facilitate the analysis of protein samples.

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Lab products found in correlation

Acetonitrile acn lc ms grade, by Biosolve (1 mentions) Acetonitrile acn, by RCI Labscan (1 mentions) Tween 20, by Merck Group (1 mentions) Triton x 100, by Merck Group (1 mentions)

Close spelling variants of this product

Sequencing grade modified (3 citations)

2 protocols using sequencing grade modified trypsinwas

1

Recombinant Human sRAGE Protocol

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Recombinant human sRAGE (rh-sRAGE;
cat. no. C423; UniProtKB ID Q15109; Ala23-Ala344 with C-terminal hexa-histidine
tag) was purchased from Novoprotein (Summit, NJ), anti-sRAGE affimers
(raised against recombinant human sRAGE from Novoprotein, cat. no.
C423) were produced and supplied by Avacta Life Sciences (Wetherby,
U.K.), and stable-isotope-labeled RAGE peptides (i.e., IGEPLVLK* and VLSPQGGGPWDSVAR*) were synthesized by Pepscan Presto (Lelystad, The Netherlands).
Acetonitrile (ACN; LC–MS grade) was obtained from Biosolve
(Valkenswaard, The Netherlands), and sequencing grade modified trypsin
was purchased from Promega (Madison, WI). Nunc-Immuno MicroWell 96-well
plates with MaxiSorp coating (cat. no. M9410), bovine serum albumin
(BSA; cat. no. A7638), Trizma base (tris; cat. no. T6791), and phosphate-buffered
saline (PBS; 10×; cat. no. D1408) as well as all other chemicals
were purchased from Sigma-Aldrich (Zwijndrecht, The Netherlands).
Klont F., Hadderingh M., Horvatovich P., ten Hacken N.H, & Bischoff R. (2018). Affimers as an Alternative to Antibodies in an Affinity LC–MS Assay for Quantification of the Soluble Receptor of Advanced Glycation End-Products (sRAGE) in Human Serum. Journal of Proteome Research, 17(8), 2892-2899.
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2

Metformin-based Cell Viability Assay

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Metformin (1,1-dimethylbiguanide
hydrochloride),
triethylammonium bicarbonate buffer (TEABC), thiazolyl blue tetrazolium
bromide (MTT), tris(2-carboxyethyl) phosphine hydrochloride (TCEP), S-methylmethanethiosulfonate, Tween 20, and Triton X-100
were purchased from Sigma-Aldrich (St. Louis, MO, USA). Acetonitrile
(ACN) was purchased from Lab-Scan. Sequencing grade modified trypsin
was purchased from Promega (Madison, WI, USA). Dimethyl sulfoxide
was purchased from Scharlau Chemie (Barcelona, Spain).
Wang W.H., Chen S.K., Huang H.C, & Juan H.F. (2021). Proteomic Analysis Reveals That Metformin Suppresses PSMD2, STIP1, and CAP1 for Preventing Gastric Cancer AGS Cell Proliferation and Migration. ACS Omega, 6(22), 14208-14219.
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