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2 protocols using phosphate buffer solution (pbs)

1

Momordica charantia Antioxidant and Anti-inflammatory Evaluation

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Momordica charantia was purchased from the local market; phosphate buffer solution (PBS), DPPH and hematoxylin–eosin were purchased from Beyotime (Shanghai, China); methanol solution, 30% H2O2 solution, FeSO4, salicylic acid, paraffin, and 4% paraformaldehyde solution were purchased from SINOPHARM (Beijing, China); dextran sodium sulfate (DSS) was purchased from MP Biomedicals (Santa Ana, CA, USA); the detection kit for CAT, SOD, LDH, and MDA was purchased from Beyotime (Shanghai, China); the detection kits for GSH and GSH-PX were purchased from Jiancheng (Nanjing, China); and the ELISA kits for IL-6, IL-10, IL-1β, and TNF-α were purchased from BOSTER (San Mateo, CA, USA).
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2

Aflatoxin Cytotoxicity Evaluation Protocol

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AFB1 and AFM1 (95% purity) were provided by Pribolab (Qingdao, Shangdong, China), and a stock liquor of 200 μM was formulated by dissolving in dimethyl sulfoxide (DMSO) (Sigma−Aldrich, St. Louis, MO, USA). RPMI 1640 and fetal bovine serum (FBS) used for cell growth were from Gibco (Grand Island, NY, USA). The antibiotics (100 U/mL penicillin and 100 μg/mL streptomycin) and nonessential amino acids (NEAAs) were obtained from Life Technologies (Carlsbad, CA, USA). L-Glutamine was supplied by Sigma-Aldrich. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) and phosphate buffer solution were provided by Beyotime Biotechnology (Shanghai, China).
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