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Scl 10a hplc

Manufactured by Shimadzu
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The SCL-10A is a high-performance liquid chromatography (HPLC) system controller manufactured by Shimadzu. It serves as the core component for managing and controlling HPLC instruments, providing essential functions for method setup, data acquisition, and analysis.

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Lab products found in correlation

Dtx 880 plate reader, by Beckman Coulter (2 mentions) Prism v 6.0b software, by GraphPad (2 mentions) A synthetic myc epitope peptide, by AnaSpec (2 mentions) Tmb elisa substrate solution, by Thermo Fisher Scientific (2 mentions)

2 protocols using scl 10a hplc

1

Quantification of MYC and CNGB3 Binding

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A synthetic MYC epitope peptide (Anaspec) was biotinylated using sulpho-NHS-biotin (Pierce) and purified using a reverse-phase high-performance liquid chromatography column eluted with a linear gradient from 30 to 70% acetonitrile in water containing 0.1% trifluoroacetate over 20 min using Shimadzu SCL-10A HPLC. Biotin-MYC was bound to streptavidin-coated 96-well plates (Pierce), and MYC levels were measured by enzyme-linked immunosorbent assay (ELISA) using rabbit anti-MYC antibody and peroxidase-conjugated anti-rabbit antibody followed by a peroxidase colour reaction with TMB ELISA substrate solution (eBioscience). Levels of CNGB-MYC expressed by CNGB3–MYC-transfected HEK293T cells were measured by ELISA using biotin-MYC as the standard. Binding of FITC-z13 to CNGB3 was measured by adding serially diluted FITC-z13 to CNGB3-expressing HEK293T cells in 96-well plates and incubation at 37 °C for 60 min. Unbound FITC-z13 was washed with PBS and fluorescence remaining in the well was quantitated using a Beckman DTX880 plate reader. Binding kinetics was analysed by GraphPad Prism v6.0b software.
Sugihara K., Kobayashi Y., Suzuki A., Tamura N., Motamedchaboki K., Huang C.T., Akama T.O., Pecotte J., Frost P., Bauer C., Jimenez JB J.r., Nakayama J., Aoki D, & Fukuda M.N. (2014). Development of pro-apoptotic peptides as potential therapy for peritoneal endometriosis. Nature Communications, 5, 4478.
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2

Quantification of MYC and CNGB3 Binding

Check if the same lab product or an alternative is used in the 5 most similar protocols
A synthetic MYC epitope peptide (Anaspec) was biotinylated using sulfo-NHS-biotin (Pierce) and purified using a reverse phase HPLC column eluted with a linear gradient from 30% to 70 % acetonitrile in water containing 0.1% trifluoroacetate over 20 min using Shimadzu SCL-10A HPLC. Biotin-MYC was bound to streptavidin-coated 96-well plates (Pierce), and MYC levels were measured by ELISA using rabbit anti-MYC antibody and peroxidase-conjugated anti-rabbit antibody followed by a peroxidase color reaction with TMB ELISA substrate solution (eBioscience). Levels of CNGB-MYC expressed by CNGB3-MYC transfected HEK293T cells were measured by ELISA using biotin-MYC as the standard. Binding of FITC-z13 to CNGB3 was measured by adding serially diluted FITC-z13 to CNGB3-expressing HEK293T cells in 96-well plates and incubation at 37°C for 60 min. Unbound FITC-z13 was washed with PBS and fluorescence remaining in the well were quantitated using a Beckman DTX880 plate reader. Binding kinetics were analyzed by GraphPad Prism v6.0b software.
Sugihara K., Kobayashi Y., Suzuki A., Tamura N., Motamedchaboki K., Huang C.T., Akama T.O., Pecotte J., Frost P., Bauer C., Jimenez JB J.r., Nakayama J., Aoki D, & Fukuda M.N. (2014). Development of pro-apoptotic peptides as potential therapy for peritoneal endometriosis. Nature Communications, 5, 4478.
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