Well ultra low binding black clear spheroid plates

Manufactured by Corning

Sourced in United States

The Well ultra-low-binding black/clear spheroid plates are a type of lab equipment designed for cell culture applications. These plates feature a specialized surface treatment that minimizes the adhesion of cells, proteins, and other biomolecules, allowing for the formation and maintenance of spheroid cultures. The plates are available in both black and clear variants to accommodate different experimental requirements and imaging needs.

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Lab products found in correlation

384 well microplate, by Corning (2 mentions) Incucyte zoom instrument, by Sartorius (2 mentions)

Spelling variants of this product

Ultra-low binding plates (9 citations) Low binding plates (3 citations) Ultra Low plates (3 citations)

2 protocols using well ultra low binding black clear spheroid plates

Proliferation and Differentiation Assays

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Coated (with poly-L-ornithine and human fibronectin) and uncoated standard 384 well microplates (Cat# 3765, Corning Inc., Corning NY, USA) and u-shaped 384 well ultra-low-binding black/clear spheroid plates (Cat# 3830, Corning Inc.) were used for assessing cell proliferation and differentiation in three different culture platforms. To assess proliferation, 5000 LUHMES cells were seeded in 80 µL of proliferation media per well, then grown for 4 days without changing media. To assess cell differentiation, 10000 cells were seeded in 80 µL of differentiation media per well and monitored for 8 days. Every other day, forty mL media were removed and replaced with fresh media using a hand-held pipettor. Phase contrast images were scanned daily with an IncuCyte ZOOM instrument (Sartorius AG, Gottingen, Germany) using a 10x objective and followed by ATP cell viability assay as described below.

Tong Z.B., Huang R., Braisted J., Chu P.H., Simeonov A, & Gerhold D.L. (2024). 3D-Suspension culture platform for high throughput screening of neurotoxic chemicals using LUHMES dopaminergic neurons. SLAS discovery : advancing life sciences R & D, 29(3).

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Microplate-Based Cell Proliferation and Differentiation

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Coated (with poly-L-ornithine and human fibronectin) and uncoated standard 384 well microplates (Cat# 3765, Corning Inc., Corning NY, USA) and u-shaped 384 well ultra-low-binding black/clear spheroid plates (Cat# 3830, Corning Inc.) were used for assessing cell proliferation and differentiation in three different culture platforms. To assess proliferation, 5000 LUHMES cells were seeded in 80 µl of proliferation media per well, then grown for 4 days without changing media. To assess cell differentiation, 10000 cells were seeded in 80 µl of differentiation media per well and monitored for 8 days. Every other day, forty µL media were removed and replaced with fresh media using a hand-held pipettor. Phase contrast images were scanned daily with an IncuCyte ZOOM instrument (Sartorius AG, Gottingen, Germany) using a 10x objective and followed by ATP cell viability assay as described below.

Netlines. (2001). BMJ : British Medical Journal, 322(7283), 405.

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