Tissue samples of the middle section of adult leaves were collected and fixed in Formalin‐Acid‐Alcohol (ethanol (>90%) 50%, glacial acetic acid 5%, formalin (37% formaldehyde) 10%). Fixed samples were infiltrated in gradual increases to 30% sucrose solution, embedded in OCT compound (Sakura Finetek USA) and frozen into cryoblocks. 20 µm block face sections were collected on 1% polyethylenimine (PEI) coated slides, stained with 0.1% calcofluor white (aq., Sigma Aldrich) for five minutes followed by 0.01% Fluorol Yellow (Santa Cruz Biotechnology) in lactic acid solution for 30 min, mounted in Vectashield anti‐fade mounting medium (VECTOR Laboratories), and sealed underneath a coverslip by nail polish. Images were captured on a Zeiss LSM 880 Confocal with FAST Airyscan using Plan‐Apochromat 10X/0.45 M27, Plan‐Apochromat 20x/0.8 M27, Plan‐Apochromat 63x/1.4 Oil DIC M27 objectives set at 515 nm emission/488 nm excitation wavelength for Fluorol Yellow and 450 nm emission/405 nm excitation wavelength for calcofluor white. Collected images were processed through superresolution Airyscan and composite pictures were processed through ImageJ. Cell counts of epidermal cell types were done with ImageJ.
Fluorol yellow
Manufactured by Zeiss
Fluorol Yellow is a fluorescent dye used in microscopy and analytical applications. It is a water-soluble, bright yellow dye that exhibits strong fluorescence when excited by ultraviolet or blue light. Fluorol Yellow can be used to label and visualize various biological samples, such as cells, tissues, and proteins.
Automatically generated - may contain errors
Lab products found in correlation
Oct compound, by Sakura Finetek (2 mentions)
Lsm 880, by Zeiss (2 mentions)
Vectashield antifade mounting medium, by Vector Laboratories (2 mentions)
Calcofluor white, by Zeiss (2 mentions)
Plan apochromat 20x 0.8 m27, by Zeiss (2 mentions)
Fluorol yellow, by Santa Cruz Biotechnology (2 mentions)
Plan apochromat, by Zeiss (1 mentions)
Plan apochromat 10x 0, by Zeiss (1 mentions)
Plan apochromat 63x, by Zeiss (1 mentions)
Plan apochromat 10x 0.45 m27, by Zeiss (1 mentions)
2 protocols using fluorol yellow
1
Leaf Epidermal Tissue Imaging Protocol
2
Multimodal Imaging of Plant Leaf Anatomy
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Tissue samples of the middle section of adult leaves were collected and fixed in Formalin-Acid-Alcohol (ethanol (>90%) 50 %, glacial acetic acid 5 %, formalin (37% formaldehyde) 10%).
Fixed samples were infiltrated in gradual increases to 30% sucrose solution, embedded in OCT compound (Sakura Finetek USA) and frozen into cryoblocks. 20 µm block face sections were collected on 1% polyethylenimine (PEI) coated slides, stained with 0.1% calcofluor white (aq., Sigma Aldrich) for five minutes followed by 0.01% Fluorol Yellow (Santa Cruz Biotechnology) in lactic acid solution for 30 minutes, mounted in Vectashield anti-fade mounting medium (VECTOR Laboratories), and sealed underneath a coverslip by nail polish. Images were captured on a Zeiss LSM 880 Confocal with FAST Airyscan using Plan-Apochromat 10X/0.45 M27, Plan-Apochromat 20x/0.8 M27, Plan-Apochromat 63x/1.4 Oil DIC M27 objectives set at 515nm emission/488nm excitation wavelength for Fluorol Yellow and 450nm emission/405nm excitation wavelength for calcofluor white. Collected images were processed through superresolution Airyscan and composite pictures were processed through ImageJ. Cell counts of epidermal cell types were done with ImageJ.
Fixed samples were infiltrated in gradual increases to 30% sucrose solution, embedded in OCT compound (Sakura Finetek USA) and frozen into cryoblocks. 20 µm block face sections were collected on 1% polyethylenimine (PEI) coated slides, stained with 0.1% calcofluor white (aq., Sigma Aldrich) for five minutes followed by 0.01% Fluorol Yellow (Santa Cruz Biotechnology) in lactic acid solution for 30 minutes, mounted in Vectashield anti-fade mounting medium (VECTOR Laboratories), and sealed underneath a coverslip by nail polish. Images were captured on a Zeiss LSM 880 Confocal with FAST Airyscan using Plan-Apochromat 10X/0.45 M27, Plan-Apochromat 20x/0.8 M27, Plan-Apochromat 63x/1.4 Oil DIC M27 objectives set at 515nm emission/488nm excitation wavelength for Fluorol Yellow and 450nm emission/405nm excitation wavelength for calcofluor white. Collected images were processed through superresolution Airyscan and composite pictures were processed through ImageJ. Cell counts of epidermal cell types were done with ImageJ.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!