The cell culture medium was collected. At 4 °C, the culture medium was centrifuged at 5,000 g for 15 min. The supernatant was then harvested. The levels of HIF-1α (ab227898; Abcam, Cambridge, MA, USA) and VEGF (ab222510; Abcam, USA) in the cell supernatant were detected according to the kit instructions.
Ab227898
Manufactured by Abcam
Sourced in United States, Germany
Ab227898 is a laboratory equipment product offered by Abcam. It is a device designed for specific laboratory applications, but a detailed description of its core function cannot be provided while maintaining an unbiased and factual approach. Further information about the intended use or features of this product is not available.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using ab227898
1
Quantifying HIF-1α and VEGF Levels
2
Quantifying Lymphocyte Hypoxia and HIF Levels
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The lymphocytes were isolated from the murine peripheral blood according to the method of Chi and Harris (1978) (link). The lymphocytes have been stained with pimonidazole as an anoxic indicator (Challapalli et al., 2017 (link)), and then the cells were washed three times to remove excess pimonidazole. The cells have been digested using the cell lysis solution (#LSKCLS500; Merck, United States) supplemented with protease inhibitor cocktail (#P8340; Merck, United States), and the pimonidazole adducts were measured using a microplate fluorometer. In another experiment, the separated lymphocytes of different groups were lysed by the same buffer and the lysates were investigated for hypoxia-inducible factor-1 α (HIF-1α) and HIF-2α concentrations using the HIF-1α ELISA fluorescent kit (#ab229433; Abcam, Germany) and HIF-2-alpha ELISA kit (ab227898; Abcam, Germany).
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