Thermal un- and refolding were monitored between 15°C and 95°C at 215 nm with a Chirascan-plus spectrometer (Applied Photophysics, UK) equipped with a 150-W xenon arc lamp. The heating/cooling rate was 1°C min−1. Samples contained about 15 µM Hip1 and were measured using a 1 mm quartz glass cuvette (Hellma, Germany). Traces were normalized by assuming a completely folded state for the first 15 data points and a completely unfolded state for the last five data points. The mean of three experiments is shown in bold, individual measurements are displayed in light blue/red.
1 mm quartz glass cuvette
Manufactured by Hellma
Sourced in Germany
The 1 mm quartz glass cuvette is a versatile laboratory equipment designed for precise optical measurements. With a path length of 1 millimeter, this cuvette is suitable for a variety of spectroscopic techniques, including UV-visible absorption and fluorescence spectroscopy. Constructed from high-quality quartz glass, the cuvette provides excellent optical transparency and durability.
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Lab products found in correlation
2 protocols using 1 mm quartz glass cuvette
1
Thermal Unfolding of Hip1 Protein
2
Thermal (Un)folding of Hip1 Protein
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Thermal un-and refolding was monitored between 15°C and 95°C at 215 nm with a Chirascanplus spectrometer (Applied Photophysics, UK) equipped with a 150-W xenon arc lamp. The heating/cooling rate was 1°C/min. Samples contained about 15 µM Hip1 and were measured using a 1 mm quartz glass cuvette (Hellma, Germany). Traces were normalized by assuming a completely folded state for the first 15 data points and a completely unfolded state for the last 5 data points. The mean of three experiments is shown in bold, individual measurements are displayed on light blue/red.
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