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Luminex magnetic bead assay

Manufactured by R&D Systems
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Sourced in United Kingdom

The Luminex magnetic bead assay is a multiplex platform that enables the simultaneous measurement of multiple analytes in a single sample. The assay utilizes color-coded magnetic beads coated with capture antibodies specific to different target proteins. The beads are mixed with the sample, and the target proteins bind to their respective capture antibodies. A reporter antibody is then added, forming an antibody sandwich. The beads are then analyzed using a Luminex analyzer, which measures the fluorescent signal generated by the reporter antibody to quantify the concentration of each target protein.

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Lab products found in correlation

Anti protease cocktail, by Roche (1 mentions) Duoset elisa kit, by R&D Systems (1 mentions) Luminex magpix analyzer, by Merck Group (1 mentions)

Close spelling variants of this product

Luminex magnetic bead-based assays Luminex Bead Assay Magnetic Luminex Assay Luminex beads Magnetic beads Luminex assay

3 protocols using luminex magnetic bead assay

1

Luminex Assay for Cytokine Detection

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A Luminex magnetic bead assay was purchased from R&D Systems, Abingdon UK which was able to detect IL-6, IL-8, IL-10 and M-CSF. The assay was performed on conditioned media collected from cells treated with cytokines for 24 h according to manufacturer’s instructions to assay protein levels in conditioned media from cytokine-treated MCs. The plate was read using a Merck Millipore Luminex MAGPIX® analyser.
Wright R.D., Dimou P., Northey S.J, & Beresford M.W. (2019). Mesangial cells are key contributors to the fibrotic damage seen in the lupus nephritis glomerulus. Journal of Inflammation (London, England), 16, 22.
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2

Cytokine and Chemokine Profiling in Tumor Lysates

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Total cell lysates were prepared from the powderized tumors as for the RNA extraction but lysed in cold RIPA buffer containing anti-protease cocktail Complete Roche, 1mM sodium orthovanadate, 1mM sodium fluoride. Tumor lysates were vortexed and incubated on ice for 20 min and then centrifuged at 14000 rpm for 15 min. Supernatants were quantified with Bradford method and 10 μg of protein lysates were run for the R&D luminex magnetic bead assay with a custom configuration of 19 cytokines and chemokines (listed on the website as: HGF, TNFA, IL1B, IL1A, IFNG, GCSF, MCSF, EPO, LIX, MDC, KC, MCP-1, RANTES, IL6, Lipocalin2, MIP1A, MIP1B, IGF1, Eotaxin). Quantitation of IL1RA was performed using the ELISA Duo set kit from R&D Systems. Data are expressed as picograms per ml ± SD.
Mascia F., Schloemann D.T., Cataisson C., McKinnon K.M., Krymskaya L., Wolcott K.M, & Yuspa S.H. (2016). Cell autonomous or systemic EGFR blockade alters the immune-environment in squamous cell carcinomas. International journal of cancer, 139(11), 2593-2597.
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3

Cytokine Profiling of Podocytes

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A Luminex magnetic bead assay was purchased from R&D Systems, Abingdon UK, which was able to detect TNF-α, IL-6, IL-8, interferon-γ-inducible protein (IP)-10, IL-10, VEGF, GM-CSF, and M-CSF. The assay was performed according to manufacturer’s instructions to assay protein levels in conditioned media from cytokine-treated podocytes. The plate was read using a Merck Millipore Luminex MAGPIX analyzer.
Wright R.D, & Beresford M.W. (2018). Podocytes contribute, and respond, to the inflammatory environment in lupus nephritis. American Journal of Physiology - Renal Physiology, 315(6), F1683-F1694.
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