ScanR high content imager (Olympus Corporation, Tokyo, Japan) equipped with a Hamamatsu ORCA-ER CCD digital camera (Hamamatsu Photonics, Hamamatsu City, Japan) was used to analyze cell-based screen results. Live cell phase-contrast imaging was performed with an IncuCyte live-cell imager (Essen Instruments Ltd., Hertfordshire, UK). A Zeiss inverted 200M microscope (Zeiss GmbH, Jena, Germany) equipped with a Hamamatsu ORCA-ER camera and MetaMorph software (Molecular Devices, Downingtown, USA) was used for analysis of kinetochore protein levels. Kinetochore intensities were quantified from maximum projections created from a Z-stack of images acquired every 0.5 μm. Zeiss Axiovert 200M microscope equipped with spinning disk CSU22 confocal scanner (Yokogawa, Tokyo, Japan) and SlideBook 5.0 software (Intelligent Imaging Innovations, Inc. Denver, CO, USA) was used for acquiring images of 3D cultures, for the analysis of kinetochore distances and live-cell imaging of Hela H2B-GFP and GFP-Spc24 HeLas with environmental control.
Hamamatsu orca er ccd digital camera
Manufactured by Hamamatsu Photonics
Sourced in Japan
The Hamamatsu ORCA-ER CCD digital camera is a high-performance camera that utilizes a cooled charge-coupled device (CCD) sensor. It is designed for applications requiring low-light detection and high image quality.
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Lab products found in correlation
Scanr high content imager, by Olympus (1 mentions)
Axiovert 200m microscope, by Yokogawa (1 mentions)
Metamorph software, by Molecular Devices (1 mentions)
Axiovert 200m microscope, by Zeiss (1 mentions)
Metamorph image analysis software, by Molecular Devices (1 mentions)
Hamamatsu orca er ccd camera, by Hamamatsu Photonics (1 mentions)
Scanr microscope, by Olympus (1 mentions)
2 protocols using hamamatsu orca er ccd digital camera
1
Multimodal Microscopy Techniques for Cell Imaging
2
Visualizing Mitotic Cell Structures
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To visualize the microtubules, pericentrin and DNA, images were acquired using ScanR microscope (Olympus Corp., Tokyo, Japan) equipped with a Hamamatsu ORCA-ER CCD digital camera (Hamamatsu Photonics, Hamamatsu City, Japan). Image stacks (10 layers at 1.0 µm step size) were captured using a 20× objective. The mitotic index (MI) was quantitated in a population of a minimum of 500 cells per treatment condition. Higher resolution images were acquired using Zeiss Axiovert 200 M microscope (Carl Zeiss Corp., Oberkochen, Germany) equipped with a Hamamatsu ORCA-ER CCD camera (Hamamatsu Photonics) and Metamorph image analysis software (Molecular Devices). Image stacks (15–35 layers at 0.2–1.0 µm step size) were captured using a 63× oil immersion objective. Image stack was converted to maximum projections before the analysis of cell morphology.
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