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H4 hybrid multi mode microplate reader

Manufactured by Agilent Technologies
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Sourced in United States

The H4 Hybrid Multi-Mode microplate reader is a versatile laboratory instrument designed for various detection modes. It can perform absorbance, fluorescence, and luminescence measurements on microplates. The device is capable of reading 6- to 384-well microplates.

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Lab products found in correlation

A22066, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Microplate reader (6925 citations) Synergy H4 Hybrid Multi-Mode Microplate Reader (158 citations) BioTek Synergy H4 Hybrid Multi-Mode Microplate Reader (5 citations) Hybrid H4 Reader (2 citations)

2 protocols using h4 hybrid multi mode microplate reader

1

Resazurin-based Cell Viability Assay

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Resazurin is a cell health indicator uses the reducing power of live cell and converts to resorufin. Resazurin is a blue, non-toxic, non-fluorescent and cell permeable compound that converts to red in color and highly fluorescent resorufin in live cells(16 (link)). About 104 B16F10 melanoma cells were seeded in each well of 96-microwell plate and treated with various concentrations of extracts and essential oil of P. atlantica subsp. mutica (0.2-200 μg/mL). After 4 h incubation, the absorbance of resazurin and resorufin was measured at 570 nm and 600 nm using H4 Hybrid Multi-Mode microplate reader (BioTek, Winooski, USA). Each experiment was done in triplicate. The half maximal inhibitory concentration (IC50) was calculated using GraphPad software from the concentration-effect curve: log concentration vs response.
Eghbali-Feriz S., Taleghani A., Al-Najjar H., Emami S.A., Rahimi H., Asili J., Hasanzadeh S, & Tayarani-Najaran Z. (2018). Anti-melanogenesis and anti-tyrosinase properties of Pistacia atlantica subsp. mutica extracts on B16F10 murine melanoma cells. Research in Pharmaceutical Sciences, 13(6), 533-545.
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2

Heteronemin-Induced ATP Measurement

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ATP determination was achieved following the manufacturer’s protocol (Invitrogen™ cat: A22066, Carlsbad, CA, USA). After treatment with heteronemin (0.15 μg/mL), cells were lysed with RIPA lysis buffer and centrifuged at 18,000 rpm for 30 min, the supernatant was separated to determine protein concentration by BCA protein assay kit. A reconstituted buffer was used containing dH2O, 20× Reaction Buffer, 0.1 M DTT, 10 mM d-luciferin, 12.5 ng firefly luciferase solution and cell lysate. Luminescence was analyzed with H4 Hybrid Multi-Mode Microplate Reader (BioTek, Winooski, VT, USA).
Chen Y.C., Lu M.C., El-Shazly M., Lai K.H., Wu T.Y., Hsu Y.M., Lee Y.L, & Liu Y.C. (2018). Breaking down Leukemia Walls: Heteronemin, a Sesterterpene Derivative, Induces Apoptosis in Leukemia Molt4 Cells through Oxidative Stress, Mitochondrial Dysfunction and Induction of Talin Expression. Marine Drugs, 16(6), 212.
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