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Anti brd4 h 250

Manufactured by Santa Cruz Biotechnology
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Anti BRD4 (H-250) is a primary antibody that specifically recognizes the BRD4 protein. BRD4 is a member of the bromodomain and extraterminal (BET) family of proteins and plays a role in transcriptional regulation. This antibody can be used for applications such as Western blotting and immunohistochemistry.

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Lab products found in correlation

Anti tubulin, by Abcam (2 mentions) Anti ac histone h4, by Santa Cruz Biotechnology (2 mentions) Anti ac histone h3k4, by Merck Group (2 mentions) Cbp a 22 sc, by Santa Cruz Biotechnology (2 mentions) Anti ac histone h3k122, by Abcam (2 mentions) Anacardic acid, by Abcam (2 mentions) Curcumin and garcinol, by Abcam (2 mentions) Jq1 inhibitor, by BPS Biosciences (2 mentions) Anti histone h3, by Cell Signaling Technology (2 mentions)

2 protocols using anti brd4 h 250

1

Histone Acetylation Antibody Validation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies used for either immunoprecipitation or immunoblotting were Anti BRD4 (H-250) :sc-48772 and anti-Ac Histone H4: sc-34263 (Santa Cruz Biotech), anti-Ac Histone H3K4 (#07-539; Millipore), anti-Ac Histone H3 (#06-599; Upstate), anti-Ac Histone H3K9, H3K14, H3K18, H3K27, H3K56, anti-Histone H3 (from #9927 kit, Cell Signaling) and anti-Histone H4 (F-9: Santa Cruz Biotech), anti-Ac Histone H4K5, H4K8, H4K12, and H4K16 (from #17-211 kit, Millipore), anti-Tubulin (ab6046; Abcam), CBP (A-22: sc-369, Santa Cruz Biotech), p300 (3G230/NM-11, ab-14984, Abcam) and anti-Ac Histone H3K122 (ab33309; Abcam). The validation of specificity of the anti-Ac Histone H3K122 antibody (Lot #436839 and #GR14667-1) and other antibodies used in the study are provided on the manufacturers’ websites. HAT inhibitors Anacardic acid, Curcumin and Garcinol (Biovision) and C646 (Sigma) were dissolved in dimethyl sulfoxide (DMSO) to make concentrated stock solutions. Stock solutions of all HAT inhibitors were stored at −80°C. The (+)-JQ1 inhibitor (BPS Bioscience) was similarly dissolved in DMSO and stored.
Devaiah B.N., Case-Borden C., Gegonne A., Hsu C.H., Chen Q., Meerzaman D., Dey A., Ozato K, & Singer D.S. (2016). BRD4 is a Histone Acetyltransferase that Evicts Nucleosomes from Chromatin. Nature structural & molecular biology, 23(6), 540-548.
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2

Histone Acetylation Antibody Validation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies used for either immunoprecipitation or immunoblotting were Anti BRD4 (H-250) :sc-48772 and anti-Ac Histone H4: sc-34263 (Santa Cruz Biotech), anti-Ac Histone H3K4 (#07-539; Millipore), anti-Ac Histone H3 (#06-599; Upstate), anti-Ac Histone H3K9, H3K14, H3K18, H3K27, H3K56, anti-Histone H3 (from #9927 kit, Cell Signaling) and anti-Histone H4 (F-9: Santa Cruz Biotech), anti-Ac Histone H4K5, H4K8, H4K12, and H4K16 (from #17-211 kit, Millipore), anti-Tubulin (ab6046; Abcam), CBP (A-22: sc-369, Santa Cruz Biotech), p300 (3G230/NM-11, ab-14984, Abcam) and anti-Ac Histone H3K122 (ab33309; Abcam). The validation of specificity of the anti-Ac Histone H3K122 antibody (Lot #436839 and #GR14667-1) and other antibodies used in the study are provided on the manufacturers’ websites. HAT inhibitors Anacardic acid, Curcumin and Garcinol (Biovision) and C646 (Sigma) were dissolved in dimethyl sulfoxide (DMSO) to make concentrated stock solutions. Stock solutions of all HAT inhibitors were stored at −80°C. The (+)-JQ1 inhibitor (BPS Bioscience) was similarly dissolved in DMSO and stored.
Devaiah B.N., Case-Borden C., Gegonne A., Hsu C.H., Chen Q., Meerzaman D., Dey A., Ozato K, & Singer D.S. (2016). BRD4 is a Histone Acetyltransferase that Evicts Nucleosomes from Chromatin. Nature structural & molecular biology, 23(6), 540-548.
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