Petunia volatiles were collected from infiltrated flowers by a closed-loop stripping method and analyzed by GC-MS (Widhalm et al., 2015 ). Briefly, floral scents were absorbed in VOC collection traps containing 20 mg of Porapak Q (80–100 mesh) matrix (Waters). VOCs were eluted from scent collection traps with 200 μl of dichloromethane. A 1 μg aliquot of naphthalene was added to the eluted sample as an internal standard. Samples were analyzed on an Agilent 6890N-5975B GC-MS system. Quantitation of different volatile compounds was performed based on standard curves generated with commercially available standards.
6890n 5975b gc ms system
Manufactured by Agilent Technologies
The 6890N-5975B GC-MS system is a gas chromatography-mass spectrometry instrument designed for analytical applications. It combines a 6890N gas chromatograph with a 5975B mass spectrometer to provide separation, identification, and quantification capabilities for a wide range of analytes.
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Lab products found in correlation
2 protocols using 6890n 5975b gc ms system
1
Petunia Floral Volatiles Analysis
2
Petunia Floral Scent Profiling
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Petunia volatiles were collected from infiltrated flowers by a closed-loop stripping method and analyzed by GC-MS (Widhalm et al., 2015) . Briefly, floral scents were absorbed in VOC collection traps containing 20 mg of Porapak Q (80-100 mesh) matrix (Waters). VOCs were eluted from scent collection traps with 200 μl dichloromethane. One microgram of naphthalene was added to the eluted sample as an internal standard. Samples were analyzed on an Agilent 6890N-5975B GC-MS system. Quantitation of different volatile compounds was performed based on standard curves generated with commercially available standards.
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