Pure substance pradofloxacin was obtained from Bayer Animal Health (Elanco, as of 2020) and prepared as per the manufacturer’s instructions. Ampicillin (Auro Pharma, Woodbridge, ON, Canada), cephalexin (Novapharm, Scarborough, ON, Canada), marbofloxacin (Zoetis, Kirkland, QC, Canada), and trimethoprim/sulfamethoxazole (TMP/SMX) (Sigma Aldrich, Oakville, ON, Canada) were purchased commercially and prepared in accordance with the manufacturer’s directions. Fresh stock solutions or samples stored at −70 °C were used for each experiment.
Trimethoprim sulfamethoxazole tmp smx
Manufactured by Merck Group
Sourced in Canada, Sao Tome and Principe
Trimethoprim/sulfamethoxazole (TMP/SMX) is a combination antibiotic medication used to treat various bacterial infections. It consists of two active ingredients, trimethoprim and sulfamethoxazole, which work together to inhibit the growth and reproduction of bacteria.
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Lab products found in correlation
2 protocols using trimethoprim sulfamethoxazole tmp smx
1
Antimicrobial Substance Preparation Protocol
2
Pneumococcal Isolate Identification and Antimicrobial Susceptibility
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Once in the laboratory, pneumococcal isolates were reidentified by standard procedures that included tests for bile solubility and optochin sensitivity. Pneumococcal isolates were serotyped by the Quellung reaction with type-and factor-specific antisera (Statens Serum Institut, Copenhagen, Denmark). Antimicrobial susceptibility tests for penicillin, cefotaxime, vancomycin, erythromycin, chloramphenicol and trimethoprim/sulfamethoxazole (TMP/SMX) all from Sigma-Aldrich, (St Louis MO,US) were performed by the broth microdilution method to determine the minimum inhibitory concentration (MIC) and clinical interpretation according to the procedures of the Clinical and Laboratory Standards Institute (CLSI) using cation-adjusted Mueller-Hinton broth (CAMHB) supplemented with 3% lysed horse blood. Interpretative criteria were differentiated for meningitis and non-meningitis isolates to penicillin and cefotaxime according to CLSI. S. pneumoniae ATCC 49619 was used as the control strain. 5
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