MOG35-55 peptide (MEVGWYRSPFSRVVHLYRNGK) was purchased from Protein and Nucleic Acid Core Facility Stanford University, Stanford, CA, USA. Pertussis toxin was purchased from List Biologicals, St. Louis, MO, USA. Methocel was purchased from Sigma Aldrich, St. Louis, MO, USA. Rabbit anti-MBP Ab, rat anti-neurofilament Ab, rabbit Anti-Ym-1 Ab, rabbit anti-iNOS Ab for immunohistology were purchased from Abcam, Cambridge, MA, USA. Rat anti-CD68 Ab was purchased from Biorad, Hercules, CA, USA. Mouse anti-GFAP-Alexa fluor 488 Ab was purchased from Biolegend, San Diego, CA, USA. Rat anti-C3 Ab was purchased from Novus Biologicals, Littleton, CO, USA.
Donkey anti-rabbit IgG Alexa 488, goat anti-rat IgG Rhodamine, goat anti-rabbit IgG Rhodamine, and chicken anti-rat IgG Alexa488 were purchased from Jackson Immunoresearch Laboratories, Inc., West Grove, PA, USA. Antibodies against F4/80, iNOS, and CD206 for flow cytometry and ELISA kits for IFN-γ, IL-17A, GM-CSF and IL-10 were purchased from eBioscience, San Diego, CA, USA.
Donkey anti-rabbit IgG Alexa 488, goat anti-rat IgG Rhodamine, goat anti-rabbit IgG Rhodamine, and chicken anti-rat IgG Alexa488 were purchased from Jackson Immunoresearch Laboratories, Inc., West Grove, PA, USA. Antibodies against F4/80, iNOS, and CD206 for flow cytometry and ELISA kits for IFN-γ, IL-17A, GM-CSF and IL-10 were purchased from eBioscience, San Diego, CA, USA.