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Antibody specific to β actin

Manufactured by Merck Group
Sourced in United States

The Antibody specific to β-actin is a laboratory reagent used for the detection and quantification of the β-actin protein. β-actin is a widely expressed cytoskeletal protein that is commonly used as a reference or control in various molecular and cellular biology experiments. This antibody can be used in techniques such as Western blotting, immunohistochemistry, and immunocytochemistry to identify and analyze the expression levels of β-actin in different samples.

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2 protocols using antibody specific to β actin

1

Caveolin-1 and PTEN Regulation

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Chemicals for preparation of culturing medium were purchased from Sigma (St. Louis, including dBcAMP and fluoxetine were purchased from Sigma (St. Louis, MO, USA).
Antibodies specific to Cav-1 antibody and PTEN were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Antibodies specific to p-AKT, AKT, p-GSK-3β or GSK-3β were obtained from Cell Signaling Technology (Cell Signaling,USA). Antibody specific to β-actin was obtained from Sigma-Aldrich (Sigma,USA). The second antibody goat anti-mouse IgG-HRP was purchased from Promega (Madison, USA). The second antibody goat anti-rabbit IgG-HRP and Cav-1 siRNA (catalog no. sc-29942) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Scramble siRNA (5'-ACGACTAGCCTGAACTCAA-3') was synthesised by Sigma (St. Louis, MO, USA
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2

Culturing Medium Preparation and Antibody Sources

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Chemicals for preparation of culturing medium were purchased from Sigma (St. Louis, MO, USA) and horse serum from Invitrogen (Carlsbad, CA, USA). Most chemicals, including dBcAMP, CBZ and VPA were purchased from Sigma (St. Louis, MO, USA). Lithium carbonate (Li2CO3) was obtained from Shanghai Hengxin Chemical Reagent (Shanghai, China). Antibodies specific to Cav-1 antibody and PTEN were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Antibodies specific to p-AKT, AKT, p-GSK-3β or GSK-3β were obtained from Cell Signaling Technology (Cell Signaling, USA). Antibody specific to β-actin was obtained from Sigma-Aldrich (Sigma, USA). The second antibody goat anti-mouse IgG-HRP was purchased from Promega (Madison, USA). The second antibody goat anti-rabbit IgG-HRP were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Opti-MEMI and Oligofectamine were purchased from Invitrogen (Carlsbad, CA, USA). Adenosine triphosphate (ATP), nicotinamide adenine dinucleotide phosphate (NADP), hexokinase, glucose-6-phosphate dehydrogenase, and amyloglucosidase were purchased from Sigma (St. Louis, MO, USA).
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